Long non-coding RNA CRNDE deteriorates intrauterine infection-induced neonatal brain injury
| Autor: | Bing-Hong Zhang, Sai Chen, Gao-Hua Wang, Fang-Fang Lai, Cheng-Zhi Fang, Cai-Xia Yan, Chun-Hua Fu |
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| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
Male Programmed cell death Amniotic fluid H&E stain Spatial Learning Morris water navigation task Biology Andrology 03 medical and health sciences Memory Pregnancy medicine Animals Humans Molecular Biology 030304 developmental biology 0303 health sciences TUNEL assay Microglia Cell Death 030306 microbiology Uterus Brain Cell Biology Rats Up-Regulation medicine.anatomical_structure Terminal deoxynucleotidyl transferase Animals Newborn Astrocytes Brain Injuries Gene Knockdown Techniques Immunohistochemistry Cytokines Female RNA Long Noncoding |
| Zdroj: | Molecular and cellular probes. 52 |
| ISSN: | 1096-1194 |
| Popis: | Background This study aimed to test the hypothesis that long non-coding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) could exacerbate brain injury caused by intrauterine infection in neonatal rats. Methods Intrauterine infection was induced in pregnant rats by lipopolysaccharide (LPS). After delivery, newborn rats with brain injury caused by intrauterine infection were randomly divided into control, control shRNA, and CRNDE shRNA groups. CRNDE expression in serum and amniotic fluid of pregnant rats and neonatal brain tissues were determined by quantitative real-time PCR (qRT-PCR). Morris water maze (MWM) task was used to test the spatial learning and memory ability. Histological examination and apoptosis detection were performed by hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Immunohistochemistry was conducted to evaluate the activation of astrocytes and microglia. Results LncRNA CRNDE was highly expressed in serum and amniotic fluid of maternal rats and in brain tissues of offspring rats. Furthermore, shRNA-mediated CRNDE downregulation could rescue the spatial learning and memory ability, improve brain histopathological changes and cell death, and inhibit the activation of astrocytes and microglia caused by LPS. Conclusion CRNDE silencing possessed a cerebral protective effect in neonatal rats with brain injury caused by interauterine infection. |
| Databáze: | OpenAIRE |
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