Integrative Analysis of Methylome and Transcriptome Reveals the Importance of Unmethylated CpGs in Non-CpG Island Gene Activation
| Autor: | Tamar Kahan, Amichai Marx, Itamar Simon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Transcriptional Activation
Article Subject Transcription Genetic Bisulfite sequencing lcsh:Medicine Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Epigenetics of physical exercise Humans Promoter Regions Genetic Psychological repression 030304 developmental biology Genetics Regulation of gene expression 0303 health sciences General Immunology and Microbiology lcsh:R General Medicine Methylation DNA Methylation Chromatin DNA-Binding Proteins CpG site Gene Expression Regulation 030220 oncology & carcinogenesis DNA methylation CpG Islands Transcriptome Research Article |
| Zdroj: | BioMed Research International, Vol 2013 (2013) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Background. Promoter methylation is associated with gene repression; however, little is known about its mechanism. It was proposed that the repression of methylated genes is achieved through the recruitment of methyl binding proteins (MBPs) that participate in closing the chromatin. An alternative mechanism suggests that methylation interferes with the binding of either site specific activators or more general activators that bind to the CpG dinucleotide. However, the relative contribution of these two mechanisms to gene repression is not known.Results. Bioinformatics analyses of genome-wide transcriptome and methylome data support the latter hypothesis by demonstrating a strong association between transcription and the number of unmethylated CpGs at the promoter of genes lacking CpG islands.Conclusions. Our results suggest that methylation represses gene expression mainly by preventing the binding of CpG binding activators. |
| Databáze: | OpenAIRE |
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