Asiaticoside Mitigates the Allergic Inflammation by Abrogating the Degranulation of Mast Cells
| Autor: | Guang Hai Yan, Jin Shi Yang, Nan Che, Liang Chang Li, Hong Cui, Hong Mei Piao, Jing Ye, Yun Ho Choi, Jing Zhi Jiang, Ming Yu Zheng, Guang Yu Jin |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Syk Immunoglobulin E Histamine Release Cell Degranulation Allergic inflammation Rats Sprague-Dawley Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine LYN Hypersensitivity medicine Animals Mast Cells Phosphorylation Cells Cultured Mice Inbred BALB C biology Interleukin-8 NF-kappa B Degranulation Interleukin General Chemistry Mast cell Triterpenes Rats 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Immunology biology.protein Cancer research General Agricultural and Biological Sciences Histamine Signal Transduction |
| Zdroj: | Journal of Agricultural and Food Chemistry. 65:8128-8135 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | The effects of asiaticoside (AS) on allergic responses mediated by mast cells were investigated. AS showed no obvious cytotoxicity on RPMCs (rat peritoneal mast cells). AS reduced the intracellular calcium in RPMCs and deprived the histamine release and degranulation. AS also decreased the generation of antigen-induced tumor necrosis factor α, interleukin (IL)-4, IL-8, and IL-1β in RBL-2H3 cells sensitized by IgE. The suppression of AS on pro-inflammatory cytokines was related with the activation of the intracellular FcεRI and the inhibition of the nuclear factor-κB signaling pathway. In addition, AS disabled the phosphorylation of antigen-induced Syk, Lyn, Gab2, and PLCγ1, thus suppressing the downstream Akt phosphorylation and MAPKs pathways. It also increased HO-1 and Nrf2 expression time dependently. In summary, we demonstrate that AS suppresses the allergic inflammation mediated by mast cells and this effect might be mediated by FcεRI-dependent signaling pathways. |
| Databáze: | OpenAIRE |
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