Detection and molecular characterization of feline hemoplasmas in wild felid species in Iran in the Middle East
| Autor: | Séverine Tasker, Iman Memarian, Fereshteh Ghazisaeedi, Nahid Atyabi, Iraj Ashrafi Tamai, Saeid Tabatabaei, Taghi Zahraei Salehi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Bacterial Feline immunodeficiency virus Felidae 040301 veterinary sciences animal diseases Immunology Animals Wild Enzyme-Linked Immunosorbent Assay Iran Cat Diseases Microbiology Polymerase Chain Reaction Article Serology 0403 veterinary science 03 medical and health sciences Persian leopard Mycoplasma RNA Ribosomal 16S Immunology and Allergy Animals Mycoplasma Infections Feline hemoplasma Phylogeny Middle East General Veterinary biology Phylogenetic tree 04 agricultural and veterinary sciences General Medicine biology.organism_classification 16S ribosomal RNA Virology humanities Mycoplasma haemofelis 030104 developmental biology Infectious Diseases Panthera pardus saxicolor Candidatus Mycoplasma turicensis Cats |
| Zdroj: | Comparative Immunology, Microbiology and Infectious Diseases Ghazisaeedi, F, Atyabi, N, Zahraei Salehi, T, Tabatabaei, S, Ashrafi Tamai, I, Memarian, I & Tasker, S 2017, ' Detection and molecular characterization of feline hemoplasmas in wild felid species in Iran in the Middle East ', Comparative Immunology, Microbiology and Infectious Diseases, vol. 54, pp. 1-6 . https://doi.org/10.1016/j.cimid.2017.07.004 |
| ISSN: | 1878-1667 |
| Popis: | Highlights • First description of feline hemoplasma and immunodeficiency virus in endangered Persian Leopard species in Iran, Middle East. • No zoo-kept wild felids were infected. • Close sequence identity with hemoplasma isolates from domestic and wild felids in Iran and other geographical origins. • Both ‘Ca. M. haemominutum’ infected leopards were old and male and were not born nor residing in zoos. Three feline hemoplasma species exist in felids: Mycoplasma haemofelis, ‘Candidatus Mycoplasma haemominutum’, and ‘Candidatus Mycoplasma turicensis’. The aims of the study were to determine the presence of, and molecularly characterize, any hemoplasmas in wild felids, including the endangered Persian leopard in Iran, the Middle East. Blood samples were collected from 19 wild felids, including three Persian leopards. Using species-specific hemoplasma PCRs and ELISA serological testing for feline leukaemia virus and feline immunodeficiency virus (FIV), two Persian leopards were found to be infected with ‘Ca. M. haemominutum’ and were seropositive for FIV. Partial 16S rRNA gene sequences were generated for these ‘Ca. M. haemominutum’ species and subsequent phylogenetic analysis revealed 97.70% to 99.45% sequence identity with those found in domestic cats from Iran and other countries. This study confirms the presence of ‘Ca. M. haemominutum’ and concurrent FIV antibody in wild felids in Iran. This represents the first report of hemoplasma in wild felids in the Middle East as well as the first report of infection in Persian leopards. |
| Databáze: | OpenAIRE |
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