A Protocadherin-Cadherin-FLRT3 Complex Controls Cell Adhesion and Morphogenesis
| Autor: | Eunjin Koh, Michael D. Yoder, Xuejun Chen, Barry M. Gumbiner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
rho GTP-Binding Proteins
animal structures Embryo Nonmammalian Integrin Morphogenesis Xenopus lcsh:Medicine Protocadherin CHO Cells Biology Xenopus Proteins 03 medical and health sciences Xenopus laevis 0302 clinical medicine Cricetulus Cricetinae Cell Adhesion Animals Humans Immunoprecipitation lcsh:Science Cell adhesion 030304 developmental biology 0303 health sciences Multidisciplinary Cadherin Developmental Biology/Morphogenesis and Cell Biology lcsh:R Membrane Proteins biology.organism_classification Cadherins Flow Cytometry Molecular biology Transmembrane protein Protocadherins 3. Good health Cell biology Fibronectin Cell Biology/Cell Adhesion Protein Transport Gene Knockdown Techniques biology.protein lcsh:Q Cell Biology/Morphogenesis and Cell Biology 030217 neurology & neurosurgery Research Article Protein Binding |
| Zdroj: | PLoS ONE PLoS ONE, Vol 4, Iss 12, p e8411 (2009) |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND:Paraxial protocadherin (PAPC) and fibronectin leucine-rich domain transmembrane protein-3 (FLRT3) are induced by TGFbeta signaling in Xenopus embryos and both regulate morphogenesis by inhibiting C-cadherin mediated cell adhesion. PRINCIPAL FINDINGS:We have investigated the functional and physical relationships between PAPC, FLRT3, and C-cadherin. Although neither PAPC nor FLRT3 are required for each other to regulate C-cadherin adhesion, they do interact functionally and physically, and they form a complex with cadherins. By itself PAPC reduces cell adhesion physiologically to induce cell sorting, while FLRT3 disrupts adhesion excessively to cause cell dissociation. However, when expressed together PAPC limits the cell dissociating and tissue disrupting activity of FLRT3 to make it effective in physiological cell sorting. PAPC counteracts FLRT3 function by inhibiting the recruitment of the GTPase RND1 to the FLRT3 cytoplasmic domain. CONCLUSIONS/SIGNIFICANCE:PAPC and FLRT3 form a functional complex with cadherins and PAPC functions as a molecular "governor" to maintain FLRT3 activity at the optimal level for physiological regulation of C-cadherin adhesion, cell sorting, and morphogenesis. |
| Databáze: | OpenAIRE |
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