Oral gallic acid improve liver steatosis and metabolism modulating hepatic lipogenic markers in obese mice
| Autor: | Marcos Flávio Silveira Vasconcelos D’Angelo, Sérgio Henrique Sousa Santos, Alfredo Maurício Batista de Paula, Deborah de Farias Lelis, Jaciara Neves Sousa, André Luiz Sena Guimarães, Alanna Fernandes Paraíso, Renato Sobral Monteiro-Junior, João Marcus Oliveira Andrade, Juliana Pinto de Lima, Eloá Mangabeira Santos |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Aging medicine.medical_specialty medicine.medical_treatment Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Internal medicine Genetics medicine Gallic acid Molecular Biology biology Chemistry Insulin Cell Biology Metabolism medicine.disease Reverse transcription polymerase chain reaction Fatty acid synthase 030104 developmental biology Polyphenol biology.protein Steatosis 030217 neurology & neurosurgery Homeostasis |
| Zdroj: | Experimental Gerontology. 134:110881 |
| ISSN: | 0531-5565 |
| Popis: | Introduction Gallic acid (GA) is a natural endogenous polyphenol found in a variety of fruits, vegetables and wines, with beneficial effects on the energetic homeostasis. Aim The present study aimed to investigate oral gallic acid effects on liver steatosis and hepatic lipogenesis markers in obese mice evaluating new possible molecular related mechanisms. Methods Twenty-four Swiss male mice were divided into four groups and fed for 60 days with standard diet (ST), standard diet plus gallic acid (ST + GA), high-fat diet (HFD), and high-fat diet plus gallic acid (HFD + GA). We evaluated the relationship between body weight, food intake and serum levels of total cholesterol, triglycerides, insulin, aspartate and alanine transaminases. Liver histology was analyzed by hematoxylin and eosin staining. These results were accompanied by bioinformatics analyses. The acetyl-CoA carboxylase (ACC), sterol regulatory element binding protein-1 (SREBP-1) and fatty acid synthase (FAS) expression was assessed by quantitative real-time reverse transcriptase PCR (qRT-PCR). Results The main findings of the present study showed that GA reduced liver steatosis, body weight and plasma insulin levels. Analyzes of hepatic steatosis related genes expression showed that ACC and FAS mRNA were significantly suppressed in liver of HFD + GA mice. These data was corroborated by bioinformatics analysis. Conclusion These data suggest an important clinical application of GA in the prevention and treatment of liver diseases. |
| Databáze: | OpenAIRE |
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