Analysis of the role of MKK-4/Sek-1 in T cell development and apoptosis
| Autor: | Leonard I. Zon, Katherine A. Forbush, Jose Alberola-Ila, Steve D. Levin, Roger M. Perlmutter, Greg Barton |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
MAP Kinase Kinase 4
T-Lymphocytes T cell Immunology Apoptosis Mice Transgenic Thymus Gland Protein Serine-Threonine Kinases Mitogen-activated protein kinase kinase Biology Mice chemistry.chemical_compound Antigens CD T-Lymphocyte Subsets Catalytic Domain medicine Animals Immunology and Allergy fas Receptor Protein kinase A Mitogen-Activated Protein Kinase Kinases Kinase Ionomycin JNK Mitogen-Activated Protein Kinases Antibodies Monoclonal General Medicine T lymphocyte Protein-Tyrosine Kinases Flow Cytometry Precipitin Tests Molecular biology Enzyme Activation Mice Inbred C57BL medicine.anatomical_structure chemistry Calcium-Calmodulin-Dependent Protein Kinases Mutation Tetradecanoylphorbol Acetate Phosphorylation Mitogen-Activated Protein Kinases Spleen |
| Zdroj: | International Immunology. 10:1077-1082 |
| ISSN: | 1460-2377 |
| DOI: | 10.1093/intimm/10.8.1077 |
| Popis: | The stress-activated protein kinases (SAPK) are a group of dual-specificity kinases with potential roles in the control of apoptosis and proliferation. In most cells they are regulated through phosphorylation by MKK-4. We have investigated the role of MKK-4 in T cell development and function by generating transgenic animals expressing catalytically inactive MKK-4 (dMKK-4) in the thymus. Our results show that overexpression of dMKK-4 does not interfere with normal T cell development. Furthermore, expression of dMKK-4 inhibits Fas- but not phorbol ester plus ionomycin-induced activation of SAPK, suggesting that a SAPK kinase different from MKK-4 is responsible for the regulation of SAPK activation after stimulation of T cells with phorbol ester plus ionomycin. We then analyzed the effect of dMKK-4 on Fas-induced apoptosis of thymocytes. Our results show that activation of SAPK is not a necessary event in Fas-induced apoptosis of thymocytes. |
| Databáze: | OpenAIRE |
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