Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma
| Autor: | Ioana Zinuca Pavel, Gabriela Fabiola Știufiuc, Rareș Ionuț Știufiuc, Nicolae Leopold, Bogdan Culic, Radu Mărginean, Constantin Mihai Lucaciu, Gabriela Morar-Bolba, V. Toma, Mihail Buse, Romulus Tetean |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
biofluids General Chemical Engineering solid plasmonic substrates 02 engineering and technology 010402 general chemistry 01 natural sciences Silver nanoparticle Article lcsh:Chemistry symbols.namesake Breast cancer Blood plasma medicine General Materials Science Plasmon SERS Substrate (chemistry) Cancer Surface-enhanced Raman spectroscopy 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences lcsh:QD1-999 symbols MVA-SERS based cancer detection 0210 nano-technology Raman spectroscopy Biomedical engineering |
| Zdroj: | Nanomaterials Volume 10 Issue 6 Nanomaterials, Vol 10, Iss 1212, p 1212 (2020) |
| ISSN: | 2079-4991 |
| Popis: | Surface enhanced Raman spectroscopy (SERS) represents a promising technique in providing specific molecular information that could have a major impact in biomedical applications, such as early cancer detection. SERS requires the presence of a suitable plasmonic substrate that can generate enhanced and reproducible diagnostic relevant spectra. In this paper, we propose a new approach for the synthesis of such a substrate, by using concentrated silver nanoparticles purified using the Tangential Flow Filtration method. The capacity of our substrates to generate reproducible and enhanced Raman signals, in a manner that can allow cancer detection by means of Multivariate Analysis (MVA) of Surface Enhanced Raman (SER) spectra, has been tested on blood plasma samples collected from 35 healthy donors and 29 breast cancer patients. All the spectra were analyzed by a combined Principal Component-Linear Discriminant Analysis. Our results facilitated the discrimination between healthy donors and breast cancer patients with 90% sensitivity, 89% specificity and 89% accuracy. This is a direct consequence of substrates&rsquo ability to generate diagnostic relevant spectral information by performing SERS measurements on pristine blood plasma samples. Our results suggest that this type of solid substrate could be employed for the detection of other types of cancer or other diseases by means of MVA-SERS procedure. |
| Databáze: | OpenAIRE |
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