Effects of electroporation on anticancer activity of 5-FU and newly synthesized zinc(II) complex in chemotherapy-resistance human brain tumor cells
| Autor: | Sevgi Irtegun Kandemir, Yusuf Alan, Nevin Turan, Kenan Buldurun, Mehmet Esref Alkis |
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| Přispěvatelé: | Dicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı, Kandemir, Sevgi İrtegün |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Electrochemotherapy antineoplastic metal complex Zn(II) complex IC50 fluorescence microscopy Anticancer activity fluorouracil zinc 4 (2 hydroxybenzylideneamino)pyrimidin 2(1h) one Cytotoxic T cell Cytotoxicity cancer resistance antineoplastic agent MTT assay T98G cell line Chemistry Brain Neoplasms Electroporation drug cytotoxicity Hematology General Medicine unclassified drug Oncology U-118MG cell line cytotoxic agent polymerization skin fibroblast brain tumor electroporation zinc derivative 5-Fluorouracil Spectral techniques chemistry.chemical_element Antineoplastic Agents Zinc antineoplastic activity chemistry Article Dermal fibroblast Cell Line Tumor Humans controlled study human cell viability drug resistance human cell glioblastoma tumor cell line Molecular biology cell proliferation Cell culture Drug Resistance Neoplasm Zinc Compounds drug synthesis pathology |
| Popis: | Zn(II) complex of Schiff base derived from the condensation of 4-aminopyrimidine-2(1H)-one with salicylaldehyde was prepared and characterized by various physico‐chemical and spectral methods for structure determination. The cytotoxic activity of the Zn(II) complex was investigated in comparison with 5-fluorouracil (5-FU) against two different human brain tumor cell lines (T98G and U118), while primer human dermal fibroblast cells (HDF) was used as control for biocompatibility. Then, the effectiveness of electroporation (EP) on cytotoxic activities of these compounds has been examined. The cytotoxicities of the 5-FU and new Zn(II) complex, alone or in combination with electroporation, were determined by MTT assay. The Zn(II) complex showed good cytotoxicity against T98G and U118 brain tumor cell lines with IC50 = 282.47 and 297.91 μM respectively, while it was safe on HDF healthy cells with IC50 = 826.72 μM. The 5-FU exhibited less cytotoxicity compared to the Zn(II) complex against T98G (IC50 = 382.35 μM) and U118 (IC50 = 396.56 μM) tumor cell lines. The combined application of Zn (II) + EP decreased the IC50 value by 5.96-fold in T98G cells and 4.76-fold in U118 cells. EP showed a similar effect in its combined application with 5-FU, resulting in a decrease of the IC50 value of 4.22-fold in the T98G cells and 3.84-fold in the U118 cells. In a conclusion, the Zn(II) complex exhibited an anticancer potential against both brain tumor cell lines (T98G and U118) and EP greatly increased the cytotoxicity of Zn(II) complex and 5-FU on these chemotherapy-resistant cells. |
| Databáze: | OpenAIRE |
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