Development of Flow Injection Spectrophotometric Method for 1-Napthylthiourea Using Sodium Nitrite and Sulphanilic Acid Diazotization Reaction
| Autor: | Sami Ullah, Muhammad Asghar, Abdul Nabi, Mohammad Yaqoob, Samar Ali, Nusrat Munawar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Flow injection analysis
Detection limit Chromatography Article Subject Chemistry 010401 analytical chemistry Relative standard deviation 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Atomic and Molecular Physics and Optics 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound lcsh:QC350-467 0210 nano-technology Hplc method Sodium nitrite Quantitative analysis (chemistry) Alkaline hydrolysis Spectroscopy lcsh:Optics. Light |
| Zdroj: | Journal of Spectroscopy, Vol 2018 (2018) |
| ISSN: | 2314-4939 2314-4920 |
| Popis: | A simple spectrophotometric method in conjunction with flow injection analysis is developed for the quantitative analysis of 1-napthylthiourea (ANTU). The reaction is based on the alkaline hydrolysis of ANTU to 1-naphthylamine at 30°C, coupled with diazotized sulphanilic acid resulting in 4-(sulphophenylazo)-1-naphthylamine which is monitored at 495 nm. The limit of detection (S/N = 3) is 0.01 mg/L with a sampling throughput of 45/h. The method is linear over the range of 0.05–120 mg/L (R2=0.9995,n=7), with calibration equation y = 0.031x−0.018 (y = absorbance; x = mg/L) and relative standard deviation (n=3) 1.8–3.6%. Experimental variables are optimised, and the interfering effects of different pesticides, organic compounds, cations, and anions at environmentally relevant concentrations are investigated. The analysis of ANTU in spiked water samples is extracted with solid phase procedure using disposable Sep-Pak C18 cartridges, and the recovery was 93 ± 1.9–110 ± 3.0%. The results of the proposed method are compared with the HPLC method by applying the F-test and paired Student t-test at 95% confidence level. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text