Flavonoids inhibit genetic toxicity produced by carcinogens in cells expressing CYP1A2 and CYP1A1
| Autor: | J. Doehmer, A.C. Musonda, S. Lautraite, J.-K. Chipman, G.O. Edwards |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Cytochrome P-450 CYP1A2 Inhibitors DNA damage Recombinant Fusion Proteins Health Toxicology and Mutagenesis 7 8-Dihydro-7 8-dihydroxybenzo(a)pyrene 9 10-oxide Toxicology DNA Adducts chemistry.chemical_compound Cricetulus Species Specificity Cytochrome P-450 CYP1A2 Cricetinae Benzo(a)pyrene Cytochrome P-450 CYP1A1 Genetics Animals Anticarcinogenic Agents Humans Prodrugs heterocyclic compounds Chrysin Apigenin Enzyme Inhibitors Rats Wistar Lung Biotransformation Cells Cultured Genetics (clinical) Carcinogen Flavonoids Mutagenicity Tests Fibroblasts respiratory system Rats Comet assay Liver chemistry Biochemistry Carcinogens Quinolines Quercetin Comet Assay DNA DNA Damage |
| Zdroj: | Mutagenesis. 17:45-53 |
| ISSN: | 1464-3804 |
| DOI: | 10.1093/mutage/17.1.45 |
| Popis: | The effects of the flavonoids quercetin, apigenin and chrysin (10 microM) on the genetic toxicity of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and benzo[a]pyrene (BaP) was investigated at sub-cytotoxic concentrations in Chinese hamster V79 cells expressing human or rat cytochromes P450. In V79 r1A2-NH and V79 h1A1-MZ cells, none of the flavonoids increased DNA strand breaks (SB) (measured by the Comet assay) or produced detectable DNA adducts (measured by 32P-post-labelling). Neither IQ nor BaP produced DNA damage in the absence of expressed CYP1A2 or CYP1A1, respectively. DNA damage measured as SB and DNA adducts was detectable in V79 r1A2-NH cells expressing rat CYP1A2 when treated with IQ (2.5-50 microM) and this was inhibited by quercetin. Likewise, DNA damage (SB and DNA adducts) was elevated in V79 h1A1-MZ cells expressing human CYP1A1 when treated with BaP (0.1-0.5 microM) and this was inhibited by chrysin and apigenin, but not by quercetin. The specificity of CYP1A1 inhibition by chrysin and apigenin and CYP1A2 inhibition by quercetin was confirmed by ethylresorufin O-deethylase assay. |
| Databáze: | OpenAIRE |
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