Electroporation for three commonly used yeast strains for two-hybrid screening experiments

Autor:	Ming-Kai Chern, Su Fang Chien, Hsin Ying Lin, Lin Sey-En
Rok vydání:	2011
Předmět:	Strain (chemistry) Electroporation Biophysics DNA Saccharomyces cerevisiae Cell Biology Lithium acetate Biology Biochemistry Molecular biology Yeast chemistry.chemical_compound Transformation (genetics) chemistry Two-Hybrid System Techniques Molecular Biology Plasmids
Zdroj:	Analytical Biochemistry. 416:117-119
ISSN:	0003-2697
DOI:	10.1016/j.ab.2011.04.038
Popis:	The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 10 6 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0193d29727eaf25f6a302b6ce340531 https://doi.org/10.1016/j.ab.2011.04.038 Zobrazit plný text záznamu Full Text from ScienceDirect