Retinoic acid: an endogenous compound of human blood. Unequivocal demonstration of endogenous retinoic acid in normal physiological conditions
| Autor: | Marcel G.M. De Ruyter, AndréP. De Leenheer, Willy E. Lambert |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Vitamin
Chromatography Chromatography Gas fungi Extraction (chemistry) Biophysics Retinoic acid Endogeny Tretinoin Cell Biology Biochemistry High-performance liquid chromatography Mass Spectrometry chemistry.chemical_compound chemistry Sephadex medicine Humans Gas chromatography Molecular Biology Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Analytical biochemistry. 98(2) |
| ISSN: | 0003-2697 |
| Popis: | The occurrence of retinoic acid (vitamin A acid) as a normal constituent of the vitamin A reserve from the body is demonstrated. Improved methodologies based on selected peak monitoring (spm) and high-performance liquid chromatography (hplc) are used for the detection of retinoic acid in EDTA-plasma. After extensive cleanup by double-phase extraction and chromatography on Sephadex LH-20, retinoic acid is determined as its methyl derivative by spm. A different approach using double-phase extraction combined with a preextraction step and hplc is used to confirm the findings of the spm experiments. Both techniques proved the presence of retinoic acid in human plasma at a concentration of 1 to 3 ng/ml. |
| Databáze: | OpenAIRE |
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