Correction to: Autolysis of Pichia pastoris induced by cold

Autor: Rodolfo Marsch, Lourdes Villa-Tanaca, María Eugenia Hidalgo-Lara, Cipriano Chávez-Cabrera, Luis B. Flores-Cotera, Luc Dendooven, Yaneth Bartolo-Aguilar
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: AMB Express, Vol 8, Iss 1, Pp 1-2 (2018)
AMB Express
ISSN: 2191-0855
DOI: 10.1186/s13568-018-0712-2
Popis: The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes in the yeast after the growth stage could reduce protein recovery costs. This study shows that a cold-shock can be applied to induce lysis of the yeast cells. A strain of P. pastoris was constructed in which the endogenous eng gene encoding a putative endo-β-1,3-glucanase was overexpressed using the cold-shock induced promoter of the cctα gene from Saccharomyces cerevisiae. In the transgenic P. pastoris, the expression of eng increased 3.6-fold after chilling the cells from 30 to 4 °C (cold-shock stage) followed by incubation for 6 h (eng expression stage). The culture was heated to 30 °C for 6 h (ENG synthesis stage) and kept at 37 °C for 24 h (lysis stage). After this procedure the cell morphology changed, spheroplasts were obtained and cellular lysis was observed. Thus, a clone of P. pastoris was obtained, which undergoes autolysis after a cold-shock.
Databáze: OpenAIRE
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