Evaluation of the Copan Myco-TB kit for the decontamination of respiratory samples for the detection of Mycobacteria
| Autor: | Deborah De Geyter, Mieke Van der Beken, Dorien Autaers, Danny Cnudde, Denis Pierard |
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| Přispěvatelé: | Faculty of Medicine and Pharmacy, Microbiology and Infection Control, Experimental Pharmacology, Supporting clinical sciences, Basic (bio-) Medical Sciences |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Myco-TB Sensitivity and Specificity Specimen Handling 03 medical and health sciences Zephiran Humans Tuberculosis Medicine Respiratory samples Bacteriological Techniques Microscopy Chromatography business.industry fungi Direct microscopy Sputum Mycobacteria Mycobacterium tuberculosis General Medicine Human decontamination decontamination University hospital Solid medium Infectious Diseases Benzalkonium Compounds business |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 37:711-714 |
| ISSN: | 1435-4373 0934-9723 |
| DOI: | 10.1007/s10096-017-3163-9 |
| Popis: | The purpose of this study was to test a newly developed decontamination and fluidization kit for processing respiratory specimens for the detection of mycobacteria: the Myco-TB procedure (developed by Copan (Brescia, Italy)). This technique was compared with the Zephiran decontamination method in use in our hospital. Respiratory specimens (n = 387: 130 endotracheal/bronchial aspirates, 172 bronchoalveolar lavages and 55 sputa) submitted to the University Hospital of Brussels between January 2016 and March 2017 were included. All samples were divided into two aliquots: one was subjected to the Myco-TB method and one to the Zephiran technique prior to culture. The sensitivities for culture for the Zephiran technique on solid media, the Myco-TB method on solid media and Myco-TB combined with the MGIT™ system were respectively 67%, 87% and 89%. The contamination rates were 22% with both the Zephiran and Myco-TB method on solid media and only 4% with the Myco-TB kit combined with the MGIT™ system. For direct microscopy, the sensitivities of the Zephiran method and the Myco-TB method were equal (40%) when the centrifugation time was 20 min. The Myco-TB decontamination method is easy and rapid to perform. It is more sensitive for culture as compared to the Zephiran method and gives lower contamination levels when combined with the MGIT™ technique. When increasing the centrifugation step to 20 min, the sensitivity of direct microscopy is equal to the Zephiran method. |
| Databáze: | OpenAIRE |
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