Surfactant abnormalities after single lung transplantation in dogs: impact of bronchoscopic surfactant administration
| Autor: | Reinhold Schmidt, Werner Seeger, Jochen Börgermann, Alexander Olk, Andreas Günther, Fred H. Splittgerber, Markus Balser, Philipp Markart, Ivar Friedrich |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Time Factors medicine.medical_treatment Statistics as Topic Severity of Illness Index chemistry.chemical_compound Postoperative Complications Pulmonary surfactant Phospholipids medicine.diagnostic_test respiratory system Lipids medicine.anatomical_structure Anesthesia Reperfusion Injury Female Cardiology and Cardiovascular Medicine Bronchoalveolar Lavage Fluid Lung Transplantation Pulmonary and Respiratory Medicine Pulmonary Surfactant-Associated Proteins Transplantation Heterologous Lung injury Models Biological Dogs Phosphatidylcholine Bronchoscopy medicine Lung transplantation Animals Surface Tension Lung Dose-Response Relationship Drug business.industry Proteins Pulmonary Surfactants medicine.disease respiratory tract diseases Transplantation Pulmonary Alveoli Disease Models Animal Bronchoalveolar lavage chemistry Surgery business Apoproteins Reperfusion injury Biomarkers |
| Zdroj: | The Journal of thoracic and cardiovascular surgery. 127(2) |
| ISSN: | 0022-5223 |
| Popis: | Objective Disturbances of the alveolar surfactant system have been implicated in the pathogenesis of reperfusion injury. The aim of this study was to evaluate the influence of exogenous surfactant administration on surfactant properties in a model of single lung transplantation. Methods We performed heterologous, left lung transplantation (+4°C ischemia; 24 hours, Euro-Collins solution) in 6 foxhounds (untreated) and in 6 animals that received calf lung surfactant extract (Alveofact) prior to explantation (only donor lung; 50 mg/kg body weight) and immediately after onset of reperfusion (both lungs, 200 mg/kg body weight). Separate but synchronized ventilation of each lung was performed, in a volume-controlled, pressure-limited mode, with animals in prone position. Bronchoalveolar lavage fluids were collected in pretransplantation lungs (control), after 24 hours of ischemia prior to transplantation (0 hours) and 6 and 12 hours after reperfusion in both the grafts and the recipient native lungs. Results Ischemic storage per se did not provoke any changes of the surfactant system; however, severe alterations occurred within 6 hours of reperfusion, resulting in a severe loss of surface activity, including a decrease in the percentage of the large surfactant aggregate fraction, reduction of the surfactant apoproteins SP-B and SP-C, the dipalmitoyl molecular species of phosphatidylcholine and phosphatidylglycerol within the large surfactant aggregate fraction. These abnormalities were restricted to the graft, with virtually normal surfactant function and composition being found in the recipient native lung. Surfactant administration fully normalized the biochemical and largely improved the biophysical surfactant properties, alongside maintenance of lung gas exchange properties. Conclusions Severe surfactant abnormalities occur exclusively in the graft when performing separate, synchronized ventilation of each lung to attenuate ventilator-induced lung injury. Bronchoscopic surfactant administration provides protection against these abnormalities and may be a therapeutic strategy in lung transplantation. |
| Databáze: | OpenAIRE |
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