Accelerated Growth, Differentiation, and Ploidy with Reduced Proliferation of Right Ventricular Cardiomyocytes in Children with Congenital Heart Defect Tetralogy of Fallot
| Autor: | Tatyana V. Sukhacheva, Roman A. Serov, Natalia V. Nizyaeva, Artem A. Burov, Stanislav V. Pavlovich, Yulia L. Podurovskaya, Maria V. Samsonova, Andrey L. Chernyaev, Aleksandr I. Shchegolev, Alexei I. Kim, Leo A. Bockeria, Gennady T. Sukhikh |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
tetralogy of Fallot children cardiomyocytes differentiation ploidy Cx43 gap junction proliferation ultrastructure interstitial tissue QH301-705.5 Heart Ventricles Article Humans Myocytes Cardiac Biology (General) Child Cell Proliferation Cell Size Ploidies Myocardium Gap Junctions Infant Cell Differentiation General Medicine Ki-67 Antigen Case-Control Studies Child Preschool Connexin 43 Female |
| Zdroj: | Cells; Volume 11; Issue 1; Pages: 175 Cells Cells, Vol 11, Iss 175, p 175 (2022) |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells11010175 |
| Popis: | The myocardium of children with tetralogy of Fallot (TF) undergoes hemodynamic overload and hypoxemia immediately after birth. Comparative analysis of changes in the ploidy and morphology of the right ventricular cardiomyocytes in children with TF in the first years of life demonstrated their significant increase compared with the control group. In children with TF, there was a predominantly diffuse distribution of Connexin43-containing gap junctions over the cardiomyocytes sarcolemma, which redistributed into the intercalated discs as cardiomyocytes differentiation increased. The number of Ki67-positive cardiomyocytes varied greatly and amounted to 7.0–1025.5/106 cardiomyocytes and also were decreased with increased myocytes differentiation. Ultrastructural signs of immaturity and proliferative activity of cardiomyocytes in children with TF were demonstrated. The proportion of interstitial tissue did not differ significantly from the control group. The myocardium of children with TF under six months of age was most sensitive to hypoxemia, it was manifested by a delay in the intercalated discs and myofibril assembly and the appearance of ultrastructural signs of dystrophic changes in the cardiomyocytes. Thus, the acceleration of ontogenetic growth and differentiation of the cardiomyocytes, but not the reactivation of their proliferation, was an adaptation of the immature myocardium of children with TF to hemodynamic overload and hypoxemia. |
| Databáze: | OpenAIRE |
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