Methotrexate treatment provokes apoptosis of proliferating keratinocyte in psoriasis patients
| Autor: | Purushoth Ethiraj, Pushpa Gnanaraj, Haripriya Dayalan, Swapna Subramanian, Anand Thirupathi, Tamilselvi Elango |
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| Rok vydání: | 2015 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Adult Keratinocytes Male medicine.medical_specialty Adolescent Biopsy Acanthosis Apoptosis Enzyme-Linked Immunosorbent Assay Biology Keratinocyte apoptotic process Real-Time Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology 030207 dermatology & venereal diseases 03 medical and health sciences Young Adult 0302 clinical medicine Psoriasis Internal medicine medicine Humans Aged Skin Hematology medicine.diagnostic_test Gene Expression Profiling General Medicine Middle Aged medicine.disease 030104 developmental biology medicine.anatomical_structure Methotrexate Immunology Cancer research Female Dermatologic Agents Keratinocyte medicine.drug |
| Zdroj: | Clinical and experimental medicine. 17(3) |
| ISSN: | 1591-9528 |
| Popis: | Psoriasis is a chronic inflammatory skin disease characterized by hyper proliferation of keratinocytes. Recent data show that the epidermis thickening in psoriasis may be related to imbalance of homeostasis caused by abnormal apoptotic process. Maintenance of keratinocyte apoptotic process is very important in psoriasis. Methotrexate (MTX) has been used for many years to restore the normal skin in psoriasis condition. However, the exact mechanism of MTX in psoriasis condition is poorly understood. The aim of this study was to examine the role of MTX on keratinocyte apoptosis pathway in psoriasis patients. A total of 58 psoriasis vulgaris patients were recruited for this study. Nonlesional skin biopsies served as control. Skin biopsies of psoriatic patients were collected and analyzed for cytosolic, mitochondria and total cytochrome c by ELISA. Expression of caspase-9, NFκBp65, pAkt1 by western blot, real-time PCR and immunohistochemical analysis of c-FLIP protein was analyzed in nonlesional and lesional skin biopsies before (day 0) and after (at the end of 6 and 12 weeks) MTX treatment. After MTX treatment, a significant increase in cytochrome c was observed when compared with before MTX treatment in psoriasis patients (p |
| Databáze: | OpenAIRE |
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