Photoacoustic imaging of integrin-overexpressing tumors using a novel ICG-based contrast agent in mice
| Autor: | Alessia Cordaro, Martina Capozza, Federica Buonsanti, Giovanni Valbusa, Lorena Pizzuto, Alessandro Maiocchi, Luisa Poggi, Francesco Blasi, Claudia Cabella, Paolo Oliva |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Biodistribution αvβ3-Integrin genetic structures Peptidomimetic Integrin lcsh:QC221-246 Photoacoustic imaging in biomedicine Contrast agents 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Medical imaging lcsh:QC350-467 Radiology Nuclear Medicine and imaging ComputingMethodologies_COMPUTERGRAPHICS Integrin binding biology Optoacoustic imaging Indocyanine green lcsh:QC1-999 eye diseases Atomic and Molecular Physics and Optics body regions Tumor targeting 030104 developmental biology chemistry Epidermoid carcinoma 030220 oncology & carcinogenesis lcsh:Acoustics. Sound Cancer research biology.protein Photoacoustic imaging lcsh:Physics lcsh:Optics. Light Research Article |
| Zdroj: | Photoacoustics, Vol 11, Iss, Pp 36-45 (2018) Photoacoustics |
| ISSN: | 2213-5979 |
| DOI: | 10.1016/j.pacs.2018.07.007 |
| Popis: | Graphical abstract PhotoAcoustic Imaging (PAI) is a biomedical imaging modality currently under evaluation in preclinical and clinical settings. In this work, ICG is coupled to an integrin binding vector (ICG-RGD) to combine the good photoacoustic properties of ICG and the favourable αvβ3-binding capabilities of a small RGD cyclic peptidomimetic. ICG-RGD is characterized in terms of physicochemical properties, biodistribution and imaging performance. Tumor uptake was assessed in subcutaneous xenograft mouse models of human glioblastoma (U-87MG, high αvβ3 expression) and epidermoid carcinoma (A431, low αvβ3 expression). ICG and ICG-RGD showed high PA signal in tumors already after 15 min post-injection. At later time points the signal of ICG rapidly decreased, while ICG-RGD showed sustained uptake in U-87MG but not in A431 tumors, likely due to the integrin-mediated retention of the probe. In conclusion, ICG-RGD is a novel targeted contrast agents for PAI with superior biodistribution, tumor uptake properties and diagnostic value compared to ICG. |
| Databáze: | OpenAIRE |
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