Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice
| Autor: | Adrien Six, D Y Loh, Evelyne Jouvin-Marche, Patrice N. Marche, Pierre-André Cazenave |
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| Rok vydání: | 1991 |
| Předmět: |
Mice
Inbred BALB C Base Sequence cDNA library Receptors Antigen T-Cell alpha-beta Molecular Sequence Data Immunology H-2 Antigens Chromosome Mapping Context (language use) Articles Biology Polymerase Chain Reaction Molecular biology Clonal deletion Mice Thymocyte Species Specificity Cosmid Animals Immunology and Allergy Amino Acid Sequence T-Cell Receptor Beta Chain Beta (finance) Peptide sequence |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | A cDNA library of TCR beta chain transcripts from BALB/c thymocytes was constructed using anchored polymerase chain reaction (PCR). Screening of this library led to the identification of a V beta gene segment, V beta 20, structurally related to V beta 3 and V beta 17. Genomic analysis of mice displaying deletions in their V beta loci, together with mapping of cosmid clones, situated V beta 20 2.5 kb beside V beta 17. The expression of V beta 20 was estimated by PCR in mice of different H-2 and Mls types. Peripheral T cells from H-2k and H-2d mice did not express V beta 20, whereas in I-E-negative mice (C57Bl/6 and SJL), V beta 20 transcripts were detected. The lack of V beta 20 transcripts in (C57Bl/6 x CBA/J)F1, (C57Bl/6 x BALB/c)F1, and in congenic B6.H-2k mice suggests that the differential use of V beta 20 is due to an I-E-mediated clonal deletion process. The involvement of the Mls super antigens was excluded by analysis of all Mls type combinations. The nature of the V beta 20-deleting element(s) is discussed in the context of the I-E/superantigen systems controlling the expression of V beta 11 and V beta 17. |
| Databáze: | OpenAIRE |
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