Determination of acyclovir in horse plasma and body fluids by high-performance liquid chromatography combined with fluorescence detection and heated electrospray ionization tandem mass spectrometry
| Autor: | Barbara Garré, K. van der Meulen, Hans Nauwynck, Noël Desmet, P. De Backer, Siska Croubels, An Maes |
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| Rok vydání: | 2008 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray ionization Clinical Biochemistry Acyclovir Administration Oral Tandem mass spectrometry Mass spectrometry Biochemistry High-performance liquid chromatography Antiviral Agents Sensitivity and Specificity Fluorescence Analytical Chemistry Pharmacokinetics Tandem Mass Spectrometry Drug Discovery Animals Horses Infusions Intravenous Molecular Biology Ganciclovir Chromatography High Pressure Liquid Pharmacology Detection limit Chromatography Chemistry Reproducibility of Results Valine General Medicine Reference Standards Blood proteins Body Fluids Valacyclovir Linear Models Quantitative analysis (chemistry) |
| Zdroj: | Biomedical chromatography : BMC. 23(2) |
| ISSN: | 1099-0801 |
| Popis: | Two methods are presented for the determination of 'respectively' the plasma protein unbound and total concentration of acyclovir in horse plasma and body fluids: first, a liquid-liquid extraction was performed on plasma, combined with HPLC-fluorescence detection for the total plasma concentration; second a more sensitive method using high-performance liquid chromatography combined with heated electrospray ionization tandem mass spectrometry (LC-HESI-MS/MS) was described for plasma and for body fluids analysis. To obtain the unbound concentration of acyclovir in plasma, a simple deproteinization step using a Microcon filter was performed. Ganciclovir was used as an internal standard. Analysis was carried out on an Inertsil 5 ODS-3 column for the HPLC-fluorescence method. For the LC-HESI-MS/MS method a PLRP-S column was used. The limit of quantification (LOQ) for the total concentration was set at 50 and 2 ng mL(-1) for the HPLC-fluorescence method and the LC-HESI-MS/MS method, respectively. The limit of quantification for the unbound concentration was set at 5 ng mL(-1) and at 2 ng mL(-1) for body fluids. The methods were successfully used to perform pharmacokinetic and clinical studies in horses after intravenous and oral dosage of acyclovir and its prodrug valacyclovir. |
| Databáze: | OpenAIRE |
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