Molecularly imprinted polymer nanogel-based fluorescence sensing of pork contamination in halal meat extracts
| Autor: | Kazuaki Minamoto, Hirobumi Sunayama, Ryota Takeuchi, Toshifumi Takeuchi, Chehasan Cheubong, Yukiya Kitayama, Eri Takano, Shunsuke Furutani |
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| Rok vydání: | 2020 |
| Předmět: |
Meat
Swine Biomedical Engineering Biophysics Nanogels Food Contamination 02 engineering and technology Biosensing Techniques 01 natural sciences Molecular Imprinting Molecularly Imprinted Polymers Electrochemistry Fluorescence microscope Animals Detection limit Chromatography Chemistry Plant Extracts 010401 analytical chemistry Molecularly imprinted polymer food and beverages General Medicine Contamination 021001 nanoscience & nanotechnology 0104 chemical sciences Red Meat Linear range Liquid handling robot Pork Meat Cattle 0210 nano-technology Molecular imprinting Biotechnology Nanogel |
| Zdroj: | Biosensorsbioelectronics. 172 |
| ISSN: | 1873-4235 |
| Popis: | Pork contamination is a serious concern for the global halal food market because many manufacturers commonly use pork instead of beef to reduce production costs. In this study, a highly sensitive fluorescent molecularly imprinted polymer nanogel (F-MIP-NG)-based sensor was developed for rapid porcine serum albumin (PSA) detection to investigate pork contamination in halal meat extracts. F-MIP-NGs were prepared via molecular imprinting and conjugation with ATTO 647N as the fluorescent reporter molecule for the post-imprinting modification (PIM) and then immobilized on gold-coated sensor chips. For achieving rapid and easy measurement, the fluorescence response was measured using a custom-made liquid handling robot equipped with a fluorescence microscope. The fluorescence response increased with increasing PSA concentration. Under optimal conditions, the F-MIP-NG-based sensors exhibited high sensitivity, a detection limit of 40 pM, a linear range of 0.25-5 nM, and excellent affinity and selectivity towards PSA, compared to potentially interfering proteins. Moreover, it was more efficient to detect beef contamination in 1 wt% pork contamination compared to the real-time polymerase chain reaction. Collectively the good analytical performance, high rates of recovery in real meat extract samples, fast detection, and a low detection limit of pork contamination (0.1 wt%) indicated the potential of the proposed sensor for detecting PSA as a marker of pork contamination in halal meat samples. The proposed sensing system based on the MIPs would open a way to establish highly sensitive and rapid sensing systems (5 min/sample) for food analysis. |
| Databáze: | OpenAIRE |
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