CSTX-13, a highly synergistically acting two-chain neurotoxic enhancer in the venom of the spider Cupiennius salei (Ctenidae)
| Autor: | Stefan Schürch, Johann Schaller, Lucia Kuhn-Nentwig, Wolfgang Nentwig, Benno Wullschleger, Jan M. Tromp, Urs Kämpfer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
Spider Multidisciplinary biology Spider Venoms Stereochemistry Molecular Sequence Data Venom Peptide Drug Synergism Spiders Biological Sciences biology.organism_classification Cupiennius salei complex mixtures Drosophila melanogaster Biochemistry chemistry CSTX Neurotoxin Animals Amino Acid Sequence Peptide sequence |
| Popis: | The survival of the spiderCupiennius saleidepends on its hunting success, which largely relies on its immediately paralyzing multicomponent venom. Here, we report on the isolation and characterization of CSTX-13, a neurotoxic enhancer in the spider venom.De novoelucidation of the disulfide bridge pattern of CSTX-13 and the neurotoxin CSTX-1 by tandem MS revealed an identical arrangement. However, in contrast to CSTX-1, CSTX-13 is a two-chain peptide with two interchain and two intrachain disulfide bridges. Furthermore, the insecticidal activity of CSTX-13 is synergistically increased in the presence of K+ions as well as of the cytolytic peptide cupiennin 1a. We demonstrated that the weakly neurotoxic CSTX-13 enhances the paralytic activity of the neurotoxin CSTX-1 by 65% when it is administered with the latter at its entirely nontoxic physiological concentration, which is 440 times below its LD50concentration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|