Preparation of chitosan-coated liposomes as a novel carrier system for the antiviral drug Triazavirin
| Autor: | Iliya D. Shulepov, Lev V. Shaidarov, Maxim A. Mironov, Ksenia V. Kozhikhova, Maria I. Tokareva, Vladimir L. Rusinov, Andrey V. Tretiyakov, Maria N. Ivantsova |
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| Rok vydání: | 2016 |
| Předmět: |
Azoles
Carrier system Surface Properties Dispersity Pharmaceutical Science 02 engineering and technology 010402 general chemistry 01 natural sciences Antiviral Agents Chitosan chemistry.chemical_compound Drug Delivery Systems Coated Materials Biocompatible Zeta potential Particle Size Liposome Chromatography Chemistry Triazines General Medicine Triazoles 021001 nanoscience & nanotechnology 0104 chemical sciences Drug Liberation Membrane Liposomes Extrusion Particle size 0210 nano-technology Nuclear chemistry |
| Zdroj: | Pharmaceutical development and technology. 23(4) |
| ISSN: | 1097-9867 |
| Popis: | Novel method for the coating of positively charged liposomes with modified chitosan was elaborated. Liposomes were prepared by stepwise extrusion through inorganic membranes (Anotop) of 0.2 and 0.1 μm pore sizes. Chitosan derivatives were synthesized via the Ugi multicomponent reaction. Several series of liposomal compositions were produced and their properties were compared in terms of particle size, polydispersity index (PDI), zeta potential and stability. The effect of various additives was investigated and the optimal composition of the lipid film was determined. The addition of the uncharged fatty esters allowed the diameter of the liposomes obtained by extrusion to be reduced to 145-150 nm with a PDI of 0.13-0.15. The prepared liposomes were loaded with the novel antiviral drug Triazavirin and used to determine the release profile. Triazavirin was included into liposome layer as a salt with biocompatible choline derivatives of limiting fatty acids. The appropriate lipid composition was used for the preparation of a larger quantity of liposomes coated by modified chitosan. It was shown that an appropriate combination of liposomes and polysaccharide layer potentially extended colloidal stability by up to 3 months and exhibited broad functional capabilities for surface modification. |
| Databáze: | OpenAIRE |
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