Quantitation of mRNAs during mouse spermatogenesis: protamine-like histone and phosphoglycerate kinase-2 mRNAs increase after meiosis
| Autor: | Ann Salkeld, Judith Rittenhouse, James M. Kramer, Robert P. Erickson |
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| Rok vydání: | 1980 |
| Předmět: |
Male
Gel electrophoresis Phosphoglycerate kinase Messenger RNA Multidisciplinary Transcription Genetic biology Spermatid RNA Molecular biology Protamine Histones Meiosis Mice Phosphoglycerate Kinase Histone medicine.anatomical_structure biology.protein medicine Animals Protamines RNA Messenger Spermatogenesis Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 77:6086-6090 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.77.10.6086 |
| Popis: | Total RNA, prepared from immature or mature mouse testes or from spermatogenic cells separated on the basis of sedimentation velocity, was translated in vitro. Mouse protamine-like histone could be identified as an in vitro translational product when [3H]arginine was used as the label. The mRNA for protamine-like histone was detected only after meiosis; the appearance of a peak of radioactivity comigrating with protamine-like histone occurred only when RNA from mature testes or late spermatid cell fractions was translated. Phosphoglycerate kinase-2 (ATP:3-phospho-D-glycerate I-phosphotransferase, EC 2.7.2.3) was identified as an in vitro translational product by affinity chromatography followed by two-dimensional gel electrophoresis or by specific immunoprecipitation when [35S]methionine was the label. The mRNA for phosphoglycerate kinase-2 was detected only in mature testes or late spermatid cell fractions. These translational assays for protamine-like histone and phosphoglycerate kinase-2 mRNAs suggest that these messages are transcribed after meiosis. |
| Databáze: | OpenAIRE |
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