Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by Aureobasidium pullulans NAC8
| Autor: | Femi Kayode Agboola, Zainab Adenike Ayinla, Adedeji Nelson Ademakinwa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:QH426-470 lcsh:Biotechnology Mutagenesis (molecular biology technique) Microbial Biotechnology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology lcsh:TP248.13-248.65 Yeast extract General Materials Science Food science biology Plackett–Burman design Strain (chemistry) Plackett-Burman design RSM Methane sulfonate biology.organism_classification Box–Behnken design Aureobasidium pullulans lcsh:Genetics 030104 developmental biology chemistry Mutagenesis DNA fingerprinting RAPD-PCR Box-Behnken design Ethidium bromide |
| Zdroj: | Journal of Genetic Engineering and Biotechnology, Vol 15, Iss 2, Pp 345-358 (2017) Journal of Genetic Engineering & Biotechnology |
| Popis: | Strain improvement of a low fructosyltransferase-producing Aureobasidium pullulans NAC8 (Accession No. KX023301) was carried out using chemical mutagens such as ethidium bromide and ethyl methane sulfonate. The wild-type and mutant strain were distinguished using Random amplified polymorphic DNA PCR and DNA fingerprinting analysis. Plackett-Burman and Box Behnken design were statistical tools used to determine important media parameters and optimization, respectively. Phenotypically and genetically, the new improved strain was different from the wild-type. The most important media parameters from PDB influencing fructosyltransferase production were ammonium chloride, sucrose and yeast extract at p |
| Databáze: | OpenAIRE |
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