Mycobacterium tuberculosis DNA Detection Using Surface Plasmon Resonance Modulated by Telecommunication Wavelength
| Autor: | Hsin-Tsung Ho, Yen-Ta Lu, I-Fang Tsai, Shih-Hsiang Hsu, Shao-Hsi Lu, Yan-Yu Lin |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Materials science Optical fiber Analytical chemistry wavelength modulation lcsh:Chemical technology Biochemistry Article Analytical Chemistry law.invention Mycobacterium tuberculosis law lcsh:TP1-1185 Electrical and Electronic Engineering Surface plasmon resonance Instrumentation biology business.industry Laser biology.organism_classification Atomic and Molecular Physics and Optics Coupling (electronics) Wavelength surface plasmon resonance Telecommunications business Sensitivity (electronics) Biosensor |
| Zdroj: | Sensors, Vol 14, Iss 1, Pp 458-467 (2013) Sensors (Basel, Switzerland) Sensors; Volume 14; Issue 1; Pages: 458-467 |
| ISSN: | 1424-8220 |
| Popis: | A surface plasmon resonance sensor for Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) is developed using repeatable telecommunication wavelength modulation based on optical fiber communications laser wavelength and stability. MTB DNA concentrations of 1 μg/mL and 10 μg/mL were successfully demonstrated to have the same spectral half-width in the dip for optimum coupling. The sensitivity was shown to be −0.087 dB/(μg/mL) at all applied telecommunication wavelengths and the highest sensitivity achieved was 115 ng/mL without thiolated DNA immobilization onto a gold plate, which is better than the sensor limit of 400 ng/mL possible with commercial biosensor equipment. |
| Databáze: | OpenAIRE |
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