MsPG3 polygalacturonase promoter elements necessary for expression during Sinorhizobium meliloti–Medicago truncatula interaction
| Autor: | Adam Kondorosi, T. Hanh Trinh, Pascal Ratet, Miguel A. Caviedes, Javier Pérez-Hormaeche, Antonio J. Palomares, Mohammed Dary, Ignacio D. Rodríguez-Llorente |
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| Přispěvatelé: | Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Institute of Genetics, Biological Research Centre [Budapest] (BRC), Hungarian Academy of Sciences (MTA)-Hungarian Academy of Sciences (MTA) |
| Rok vydání: | 2003 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences Sinorhizobium meliloti biology Transgene food and beverages Soil Science Plant Science biology.organism_classification 01 natural sciences Molecular biology Medicago truncatula 03 medical and health sciences Start codon Gene expression Transcriptional regulation [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Medicago sativa Gene 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Plant and Soil Plant and Soil, Springer Verlag, 2003, 257 (1), pp.19-26. ⟨10.1023/A:1026219418514⟩ |
| ISSN: | 0032-079X 1573-5036 |
| DOI: | 10.1023/a:1026219418514 |
| Popis: | MsPG3 is a Medicago sativa polygalacturonase gene isolated in our laboratory, its expression during nodule development is associated with the presence of Sinorhizobium meliloti. By RT-PCR and in situhybridization studies we showed the early induction of MsPG3 expression after inoculation of M. sativaplants with the microsymbiont, suggesting a role for MsPG3 product in the early stages of the interaction. To localize its expression in nodule as well as to characterize the transcriptional regulation of MsPG3, Medicago truncatula transgenic plants containing a 2.7 kb MsPG3 promoter-β-glucuronidase (gus)gene fusion have been obtained. These transgenic plants showed nodule-specific gusexpression pattern. Five MsPG3 promoter fragments of different length (from 600 to 92 bp) fused to gusgene have been used to transform M. truncatula in order to determine the promoter regions responsible of this nodule specificity. The region between 600 and 413 bp of MsPG3 promoter was found necessary for transgenic gusexpression in nodules. This result was also confirmed in V. hirsuta transgenic `hairy roots'. At position −474/−492 bp upstream of the start codon a sequence was identified that is homologous to the binding site of the transcription factor ENBP1. Our results suggest that MsPG3 expression in nodules could be regulated through this element. |
| Databáze: | OpenAIRE |
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