Biodegradability and toxicity of monorhamnolipid biosurfactant diastereomers
| Autor: | Ricardo Palos Pacheco, Christopher I. Olivares, Joan E. Curry, Scott W. Malm, Jeanne E. Pemberton, Robert L. Tanguay, Michael T. Simonich, Robin Polt, Raina M. Maier, Anoop S. Hunjan, Fei Tian, David E. Hogan, Walter T. Klimecki |
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| Rok vydání: | 2018 |
| Předmět: |
Environmental Engineering
Embryo Nonmammalian Health Toxicology and Mutagenesis Vibrionaceae 0211 other engineering and technologies Embryonic Development 02 engineering and technology 010501 environmental sciences 01 natural sciences complex mixtures Article Cell Line Surface-Active Agents Environmental Chemistry Animals Humans Cytotoxicity Waste Management and Disposal Zebrafish 0105 earth and related environmental sciences EC50 021110 strategic defence & security studies Chromatography Chemistry Diastereomer Stereoisomerism Biodegradation Pollution Acute toxicity Congener Biodegradation Environmental Toxicity Luminescent Measurements Pseudomonas aeruginosa lipids (amino acids peptides and proteins) Ecotoxicity Glycolipids |
| Zdroj: | Journal of hazardous materials. 364 |
| ISSN: | 1873-3336 |
| Popis: | Synthetic monorhamnolipids differ from biologically produced material because they are produced as single congeners, depending on the β-hydroxyalkanoic acid used during synthesis. Each congener is produced as one of four possible diastereomers resulting from two chiral centers at the carbinols of the lipid tails [(R,R), (R,S), (S,R) and (S,S)]. We compare the biodegradability (CO2 respirometry), acute toxicity (Microtox assay), embryo toxicity (Zebrafish assay), and cytotoxicity (xCELLigence and MTS assays) of synthetic rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (Rha-C10-C10) monorhamnolipids against biosynthesized monorhamnolipid mixtures (bio-mRL). All Rha-C10-C10 diastereomers and bio-mRL were inherently biodegradable ranging from 34 to 92% mineralized. The Microtox assay showed all Rha-C10-C10 diastereomers and bio-mRL are slightly toxic according to the US EPA ecotoxicity categories with 5 min EC50 values ranging from 39.6 to 87.5 μM. The zebrafish assay showed that of 22 developmental endpoints tested, only mortality was observed at 120 h post fertilization; all Rha-C10-C10 diastereomers and bio-mRL caused significant mortality at 640 μM, except the Rha-C10-C10 (R,R) which showed no developmental effects. xCELLigence and MTS showed IC50 values ranging from 103.4 to 191.1 μM for human lung cell line H1299 after 72 h exposure. These data provide key information regarding Rha-C10-C10 diastereomers that is pertinent when considering potential applications. |
| Databáze: | OpenAIRE |
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