Increased activity of indoleamine 2,3-dioxygenase in serum from acutely infected dengue patients linked to gamma interferon antiviral function
| Autor: | Irene Bosch, Barbara A. Evans, Norma de Bosch, James E. Evans, Rajas V. Warke, Alan L. Rothman, Aniuska Becerra, Kris Xhaja, Katherine J. Martin |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Adolescent T cell viruses Dengue virus Biology medicine.disease_cause Microbiology Immune tolerance Dengue Interferon-gamma Young Adult Immune system Interferon Virology medicine Humans Indoleamine-Pyrrole 2 3 -Dioxygenase Interferon gamma Indoleamine 2 3-dioxygenase Child Cells Cultured Animal virus diseases Dendritic cell Dendritic Cells biochemical phenomena metabolism and nutrition Dengue Virus Middle Aged Up-Regulation medicine.anatomical_structure Immunology Acute Disease Female medicine.drug |
| Zdroj: | The Journal of general virology. 90(Pt 4) |
| ISSN: | 0022-1317 |
| Popis: | The depletion ofl-tryptophan (L-Trp) has been associated with the inhibition of growth of micro-organisms and also has profound effects on T cell proliferation and immune tolerance. The enzyme indoleamine 2,3-dioxygenase (IDO) catalyses the rate-limiting step in the catabolic pathway of L-Trp. Gene expression analysis has shown upregulation of genes involved in L-Trp catabolism inin vitromodels of dengue virus (DENV) infection. To understand the role of IDO during DENV infection, we measured IDO activity in sera from control and DENV-infected patients. We found increased IDO activity, lower levels of L-Trp and higher levels ofl-kynurenine in sera from DENV-infected patients during the febrile days of the disease compared with patients with other febrile illnesses and healthy donors. Furthermore, we confirmed upregulation of IDO mRNA expression in response to DENV infectionin vitro, using a dendritic cell (DC) model of DENV infection. We found that the antiviral effect of gamma interferon (IFN-γ) in DENV-infected DCsin vitrowas partially dependent on IDO activity. Our results demonstrate that IDO plays an important role in the antiviral effect of IFN-γagainst DENV infectionin vitroand suggest that it has a role in the immune response to DENV infectionsin vivo. |
| Databáze: | OpenAIRE |
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