Development and Validation of a Capillary Zone Electrophoresis Method for the Determination of Calcium in Composite Biomaterials
| Autor: | Viviana Silvia Lourdes Mouriño, Silvia Edith Lucangioli, Juan Pablo Cattalini, Javier Garcia |
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| Rok vydání: | 2014 |
| Předmět: |
CIENCIAS MÉDICAS Y DE LA SALUD
Chromatography Materials science CALCIUM QUANTITATION Composite number chemistry.chemical_element Mineralogy CAPILLARY ZONE ELECTROPHORESIS IONS RELEASE Calcium VALIDATION Biomateriales Biotecnología de la Salud INDIRECT UV Analytical Chemistry Capillary electrophoresis chemistry COMPOSITE MATERIALS purl.org/becyt/ford/3 [https] purl.org/becyt/ford/3.4 [https] |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1573-4110 |
| DOI: | 10.2174/15734110113099990038 |
| Popis: | This work presents the development and validation of a capillary zone electrophoresis method for calcium ions release quantitation from composite biomaterials with potential application in bone tissue engineering. Calcium ions quantitation was made using a fused silica capillary (40 cm, 75 µm ID) and background electrolyte containing 5 mM imidazole, 6 mM á-hydroxyisobutyric acid, 1 mM 1,4,7,10,13,16-hexaoxacyclo-octadecane and 20 % w/v methanol, at pH 4.5. Indirect UV-detection mode at 214 nm was performed. The separations were achieved using normal polarity at 6 kV, a cartridge temperature of 25 µC and 0.5 psi for 5 s for sample introduction. Parameters of validation such as specificity, linearity, limit of detection and quantitation, accuracy, precision and robustness were evaluated according to the International Conference on Harmonization guidelines. The new developed method resulted to be suitable for calcium determination from composite biomaterials for potential application in bone tissue engineering. Fil: Cattalini, Juan Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina; Fil: García, Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Fil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina; Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina |
| Databáze: | OpenAIRE |
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