Chemical PARP Inhibition Enhances Growth of Arabidopsis and Reduces Anthocyanin Accumulation and the Activation of Stress Protective Mechanisms
| Autor: | Graham Noctor, Jenny Neukermans, Per Mühlenbock, Philipp Schulz, Michael Metzlaff, Katrien Van Der Kelen, Markus Teige, Frank Van Breusegem, Matthew A. Hannah |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Sucrose Transcription Genetic Microarrays Arabidopsis lcsh:Medicine Gene Expression Secondary Metabolism POLY(ADP-RIBOSE) POLYMERASE Transcriptional control Plant Science medicine.disease_cause SECONDARY METABOLISM 01 natural sciences Biochemistry Anthocyanins Gene Expression Regulation Plant Gene expression Transcriptional regulation Vitamin C OXIDATIVE STRESS Photosynthesis Enzyme Inhibitors lcsh:Science GENE-EXPRESSION 2. Zero hunger Regulation of gene expression 0303 health sciences Multidisciplinary biology Plant Biochemistry food and beverages Metabolome DROUGHT STRESS Oxidation-Reduction Research Article Biotechnology Programmed cell death Arabidopsis Thaliana HIGH LIGHT Poly(ADP-ribose) Polymerase Inhibitors Molecular Genetics 03 medical and health sciences Model Organisms Plant and Algal Models Stress Physiological Chemical Biology medicine Oxidation-reduction reactions Gene Regulation Secondary metabolism ADP-RIBOSYLATION Biology 030304 developmental biology lcsh:R REDOX STATE Biology and Life Sciences Computational Biology 15. Life on land biology.organism_classification Biosynthetic Pathways SUCROSE-SPECIFIC INDUCTION Metabolism CELL-DEATH Seedlings Oxidative stress lcsh:Q Plant Biotechnology Plant resistance to abiotic stress 010606 plant biology & botany |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 5, p e37287 (2012) PLOS ONE |
| ISSN: | 1932-6203 |
| Popis: | Poly-ADP-ribose polymerase (PARP) post-translationally modifies proteins through the addition of ADP-ribose polymers, yet its role in modulating plant development and stress responses is only poorly understood. The experiments presented here address some of the gaps in our understanding of its role in stress tolerance and thereby provide new insights into tolerance mechanisms and growth. Using a combination of chemical and genetic approaches, this study characterized phenotypes associated with PARP inhibition at the physiological level. Molecular analyses including gene expression analysis, measurement of primary metabolites and redox metabolites were used to understand the underlying processes. The analysis revealed that PARP inhibition represses anthocyanin and ascorbate accumulation under stress conditions. The reduction in defense is correlated with enhanced biomass production. Even in unstressed conditions protective genes and molecules are repressed by PARP inhibition. The reduced anthocyanin production was shown to be based on the repression of transcription of key regulatory and biosynthesis genes. PARP is a key factor for understanding growth and stress responses of plants. PARP inhibition allows plants to reduce protection such as anthocyanin, ascorbate or Non-Photochemical-Quenching whilst maintaining high energy levels likely enabling the observed enhancement of biomass production under stress, opening interesting perspectives for increasing crop productivity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|