A simplified method for detecting Gram-positive and Gram-negative bacteria in dental histological samples: A preliminary and comparative study

Autor: Paolo Savadori, Sophia Dalfino, Marco Piazzoni, Matteo Parrini, Massimo Del Fabbro, Gianluca Martino Tartaglia, Luciano Giardino
Přispěvatelé: CTR, RS: MERLN - Complex Tissue Regeneration (CTR)
Rok vydání: 2022
Předmět:
Zdroj: Acta Histochemica, 125(1):151992. Urban und Fischer Verlag Jena
ISSN: 1618-0372
0065-1281
Popis: Objective: Bacteria identification inside the dental tissue is a complex procedure requiring specific protocols. This study aimed to compare two classical Gram staining methods with a new staining method proposed by the authors to detect Gram-positive and Gram-negative bacteria in dental histological samples of human dentin.Methods: Ten human teeth, extracted because of various pathologies, were decalcified, dehydrated, and paraffinembedded. Then, approximately 100 serial sections of 4 mu m thickness were made per sample. The serial sections were placed on glass slides and were stained according to Brown-Brenn, Brown-Hopps, and a proposed modification of Brown-Brenn staining. Both ATCC strains, smeared on glass slides, were stained following each method's instructions used in histological samples.Results: From a qualitative evaluation, the Brown-Brenn method resulted in better staining of Gram-positive bacteria, while the authors' proposed staining technique was more oriented towards Gram-negative bacteria. On the other hand, the Brown-Hopps showed quite a balance in detecting Gram-positive and Gram-negative bacteria. Unlike the Brown-Brenn stain, the other two protocols showed better stainability of Gram-negative microorganisms in bacterial-smeared samples.Conclusion: All staining techniques evaluated in this article can identify bacteria, but the outcome can change according to the staining procedure used.
Databáze: OpenAIRE
Externí odkaz: