Molecular dynamics simulations of the human CAR ligand-binding domain: deciphering the molecular basis for constitutive activity
| Autor: | Johanna Jyrkkärinne, Paavo Honkakoski, Björn Windshügel, Wolfgang Sippl, Antti Poso |
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| Rok vydání: | 2004 |
| Předmět: |
Models
Molecular Receptors Steroid Stereochemistry Molecular Sequence Data Receptors Cytoplasmic and Nuclear Ligands Calcitriol receptor Catalysis Inorganic Chemistry Molecular dynamics Coactivator Constitutive androstane receptor Humans Amino Acid Sequence Clotrimazole Physical and Theoretical Chemistry Transcription factor Constitutive Androstane Receptor Pregnane X receptor Binding Sites Chemistry Organic Chemistry Pregnane X Receptor Computational Biology Protein Structure Tertiary Computer Science Applications Gene Expression Regulation Computational Theory and Mathematics Nuclear receptor Structural Homology Protein Docking (molecular) Mutation Receptors Calcitriol Sequence Alignment Transcription Factors |
| Zdroj: | Journal of Molecular Modeling. 11:69-79 |
| ISSN: | 0948-5023 1610-2940 |
| DOI: | 10.1007/s00894-004-0227-4 |
| Popis: | The constitutive androstane receptor (CAR) belongs to the superfamily of nuclear-hormone receptors that function as ligand-activated transcription factors. CAR plays an essential role in the metabolism of xenobiotics and shows—in contrast to related receptors—constitutive activity. However, the molecular basis for the constitutive activity remains unclear. In the present study, homology models of the ligand binding domain (LBD) were generated based on the crystal structures of the related pregnane X (PXR) and the vitamin D receptor (VDR). The models were used to investigate the basal activity of CAR and the effect of coactivator binding. Molecular dynamics (MD) simulations of complexed and uncomplexed receptor revealed a hypothesis for the activation mechanism. The suggested mechanism is supported by experimental results from site-directed mutagenesis. The basal activity of CAR can be explained by specific van-der-Waals interactions between amino acids on the LBD and its C-terminal activation domain (AF-2). Docking studies with the GOLD program yielded the interaction modes of structurally diverse agonists, giving insight into mechanisms by which ligands enhance CAR activity. Figure The constitutive activity. Favorable regions of interactions between the GRID methyl probe and the AF-2 truncated LBD (colored magenta, contour level -2.5 kcal mol 1). Only the MOLCAD surface of the LBD is shown, colored according to the lipophilic potential (blue polar, brown lipophilic). The position of the two hydrophobic residues Leu343 and Ile346 from the AF-2 helix (colored cyan) is in close agreement with the GRID results. |
| Databáze: | OpenAIRE |
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