Analysis of Immunoglobulin Classes and Subclasses in Response to Infection of Balb/cJ and C57BL/6J Mice with Coxiella burnetii
| Autor: | Wolfgang Baumgärtner, R. Hofer, E. Hoffmeister, N. Schmeer, Reinhard Weiss |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Lipopolysaccharide Immunoglobulins Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Q fever Microbiology Mice chemistry.chemical_compound Immune system Antigen medicine Animals Seroconversion Mice Inbred BALB C biology General Medicine Coxiella burnetii biology.organism_classification medicine.disease Mice Inbred C57BL Disease Models Animal chemistry Immunology biology.protein Female Antibody Q Fever Hormone |
| Zdroj: | Journal of Veterinary Medicine, Series B. 44:49-59 |
| ISSN: | 1439-0450 0931-1793 |
| Popis: | Summary In order to establish defined immunological parameters for Q fever infection models, a microtitre enzyme-linked immunosorbent fluorescence assay (ELISA) was used for the first time to analyse the humoral immune response of Balb/cJ and C57BL/6J mice after experimental infection with Coxiella burnetii strain ‘Nine Mile’ in phase I. The experimental infection evoked a seroconversion in all mice within 10 days. Typically, the immune response measured against the whole-cell antigen showed an early increase of immunoglobulin (Ig) M followed by a later increase of the IgG subclasses. The IgA was low during the entire investigation period. Within the IgG subclasses only IgG2a and IgG2b gained higher values, whereby C57BL/6J mice produced high IgG2b titres and significantly lower IgG2a titres. In contrast, Balb/cJ mice developed IgG2a and IgG2b at equal levels. The use of partial antigens of C. burnetii demonstrated that the dominating IgG2b reaction of the C57BL/6J mice was directed against the lipopolysaccharide (LPS) of C. burnetii. This reaction was almost absent in Balb/cJ mice. In contrast, the SP27 protein antigen did not evoke different IgG2b reactions within the two breeds. No significant influence was observed within the two breeds in regard to sex or between hormone synchronized and non-hormone synchronized animals. |
| Databáze: | OpenAIRE |
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