Provirophages and transpovirons as the diverse mobilome of giant viruses
| Autor: | Natalya Yutin, Christelle Desnues, Ghislain Fournous, Angélique Campocasso, Sonia Monteil, Saïd Azza, Catherine Robert, Priscilla Jardot, Eugene V. Koonin, Didier Raoult, Bernard La Scola |
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| Přispěvatelé: | Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), National Center for Biotechnology Information, National Institutes of Health, Department of Health and Human Services, Bethesda, Md, National Institutes of Health [Bethesda] (NIH), Desnues, Christelle, INSB-INSB-Centre National de la Recherche Scientifique (CNRS) |
| Předmět: |
Virophages
Molecular Sequence Data Pithovirus 03 medical and health sciences Bacteriophages Mimiviridae Giant Virus [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology In Situ Hybridization Fluorescence ComputingMilieux_MISCELLANEOUS 030304 developmental biology [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology Genetics 0303 health sciences Mimivirus Multidisciplinary biology 030306 microbiology Virophage Biological Sciences biology.organism_classification Virology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology DNA Viral [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology DNA Transposable Elements Megavirus Mobilome |
| Zdroj: | HAL Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, 2012, 109 (44), pp.18078-18083 Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2012, 109 (44), pp.18078-18083 |
| ISSN: | 0027-8424 1091-6490 |
| Popis: | A distinct class of infectious agents, the virophages that infect giant viruses of the Mimiviridae family, has been recently described. Here we report the simultaneous discovery of a giant virus of Acanthamoeba polyphaga (Lentille virus) that contains an integrated genome of a virophage (Sputnik 2), and a member of a previously unknown class of mobile genetic elements, the transpovirons. The transpovirons are linear DNA elements of ∼7 kb that encompass six to eight protein-coding genes, two of which are homologous to virophage genes. Fluorescence in situ hybridization showed that the free form of the transpoviron replicates within the giant virus factory and accumulates in high copy numbers inside giant virus particles, Sputnik 2 particles, and amoeba cytoplasm. Analysis of deep-sequencing data showed that the virophage and the transpoviron can integrate in nearly any place in the chromosome of the giant virus host and that, although less frequently, the transpoviron can also be linked to the virophage chromosome. In addition, integrated fragments of transpoviron DNA were detected in several giant virus and Sputnik genomes. Analysis of 19 Mimivirus strains revealed three distinct transpovirons associated with three subgroups of Mimiviruses. The virophage, the transpoviron, and the previously identified self-splicing introns and inteins constitute the complex, interconnected mobilome of the giant viruses and are likely to substantially contribute to interviral gene transfer. |
| Databáze: | OpenAIRE |
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