Ground flaxseed reverses protection of a reduced-fat diet against Citrobacter rodentium-induced colitis
| Autor: | Kathene C. Johnson-Henry, Eberhard Lurz, Richard Y. Wu, Krista A. Power, Sumayyah Abiff, Dion Lepp, Steven R. Botts, Agostino Pierro, Philip M. Sherman, Bo Li, C. William Yeung, Marc E. Surette, Pekka Määttänen |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Physiology Inflammation digestive system Microbiology 03 medical and health sciences Mice Physiology (medical) Flax Reduced fat Citrobacter rodentium Medicine Animals Colitis Pathogen Diet Fat-Restricted 2. Zero hunger chemistry.chemical_classification Citrobacter Hepatology biology business.industry Gastroenterology Enterobacteriaceae Infections medicine.disease biology.organism_classification 3. Good health Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology chemistry Fatty Acids Unsaturated Colitis Ulcerative medicine.symptom business Dysbiosis Polyunsaturated fatty acid |
| Zdroj: | American journal of physiology. Gastrointestinal and liver physiology. 315(5) |
| ISSN: | 1522-1547 |
| Popis: | Flaxseed is high in ω-3 polyunsaturated fatty acids, fiber, and lignans known to lower cholesterol levels. However, its use for prevention or treatment of inflammatory bowel diseases has yielded mixed results, perhaps related to dietary interactions. In this study, we evaluated the impact of ground flaxseed supplementation on the severity of Citrobacter rodentium-induced colitis in the setting of either a high-fat (HF, ~36%kcal) or reduced-fat (RF, ~12%kcal) diet. After weaning, C57BL/6 mice ( n = 8–15/treatment) were fed ground flaxseed (7 g/100 g diet) with either HF (HF Flx) or RF (RF Flx) diets for 4 wk before infection with C. rodentium or sham gavage. Weight changes, mucosal inflammation, pathogen burden, gut microbiota composition, tissue polyunsaturated fatty acids, and cecal short-chain fatty acids were compared over a 14-day infection period. The RF diet protected against C. rodentium-induced colitis, whereas the RF Flx diet increased pathogen burden, exacerbated gut inflammation, and promoted gut dysbiosis. When compared with the RF diet, both HF and HF Flx diets resulted in more severe pathology in response to C. rodentium infection. Our findings demonstrate that although an RF diet protected against C. rodentium-induced colitis and associated gut dysbiosis in mice, beneficial effects were diminished with ground flaxseed supplementation. NEW & NOTEWORTHY Our results demonstrate a strong protective effect of a reduced-fat diet against intestinal inflammation, dysbiosis, and pathogen burden during Citrobacter rodentium-induced colitis. However, ground flaxseed supplementation in the setting of a reduced-fat diet exacerbated colitis despite higher levels of intestinal n-3 polyunsaturated fatty acids and cecal short-chain fatty acids. |
| Databáze: | OpenAIRE |
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