The clinical value and biological function of PTTG1 in colorectal cancer
| Autor: | Bingwei Jin, Qinggui Ren |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Oncology medicine.medical_specialty Colorectal cancer Mouse model of colorectal and intestinal cancer Metastasis 03 medical and health sciences 0302 clinical medicine Western blot Cell Line Tumor Internal medicine Biomarkers Tumor medicine Humans Neoplasm Invasiveness RNA Messenger Neoplasm Metastasis Tumor Stem Cell Assay Aged Cell Proliferation Pharmacology Oncogene medicine.diagnostic_test business.industry Cell growth Pituitary tumors General Medicine Middle Aged Prognosis medicine.disease Gene Expression Regulation Neoplastic Securin 030104 developmental biology Gene Knockdown Techniques 030220 oncology & carcinogenesis Immunohistochemistry Female Colorectal Neoplasms business |
| Zdroj: | Biomedicine & Pharmacotherapy. 89:108-115 |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2017.01.115 |
| Popis: | Pituitary tumor transforming gene-1 (PTTG1) has been suggested to serve as an oncogene in several types of human tumors, but little is known about the biological function of PTTG1 in colorectal cancer. PTTG1 mRNA and protein expressions in colorectal cancer tissues and cell lines were measured by qRT-PCR, western blot or immunohistochemistry. The association between PTTG1 protein expression and clinicopathological features was analyzed. The function of PTTG1 on colorectal cancer cell proliferation and metastasis were explored through MTT, colony formation, migration and invasion assays. In our results, PTTG1 mRNA and protein expressions were increased in colorectal cancer tissues and cell lines compared with normal colonic tissues and colon epithelial cell line. PTTG1 overexpression positively associated with clinical stage, T classification, N classification, M classification and differentiation. The univariate and multivariate analyses suggested PTTG1 overexpression was an independent poor prognostic factor for colorectal cancer patients. The in vitro experiments showed knocking down PTTG1 inhibited colorectal cancer growth and metastasis. In conclusion, PTTG1 is an independent prognostic factor and acts as an oncogene in colorectal cancer. |
| Databáze: | OpenAIRE |
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