Piwi Is Required during Drosophila Embryogenesis to License Dual-Strand piRNA Clusters for Transposon Repression in Adult Ovaries

Autor: Carolina Varela-Chavez, Abdou Akkouche, Blaise Li, Raoul Raffel, Bridlin Barckmann, Alain Pélisson, Séverine Chambeyron, Bruno Mugat
Přispěvatelé: Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Transposable element
endocrine system
Chromosomal Proteins
Non-Histone
Heterochromatin
[SDV]Life Sciences [q-bio]
Piwi-interacting RNA
Epigenetic Repression
Methylation
Germline
Histones
03 medical and health sciences
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Morphogenesis
Animals
Drosophila Proteins
RasiRNA
RNA
Small Interfering
Molecular Biology
ComputingMilieux_MISCELLANEOUS
Genetics
[SDV.GEN]Life Sciences [q-bio]/Genetics
biology
urogenital system
Ovary
Age Factors
Gene Expression Regulation
Developmental
Drosophila embryogenesis
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Cell Biology
DNA Methylation
Drosophila melanogaster
Fertility
030104 developmental biology
Histone
Argonaute Proteins
DNA Transposable Elements
biology.protein
Female
RNA Interference
Infertility
Female
Reprogramming
Protein Binding
Zdroj: Molecular Cell
Molecular Cell, Elsevier, 2017, 66 (3), pp.411-419.e4. ⟨10.1016/j.molcel.2017.03.017⟩
Molecular Cell, Elsevier, 2017, 66 (3), pp.411-419. ⟨10.1016/j.molcel.2017.03.017⟩
ISSN: 1097-2765
DOI: 10.1016/j.molcel.2017.03.017⟩
Popis: Most piRNAs in the Drosophila female germline are transcribed from heterochromatic regions called dual-strand piRNA clusters. Histone 3 lysine 9 trimethylation (H3K9me3) is required for licensing piRNA production by these clusters. However, it is unclear when and how they acquire this permissive heterochromatic state. Here, we show that transient Piwi depletion in Drosophila embryos results in H3K9me3 decrease at piRNA clusters in ovaries. This is accompanied by impaired biogenesis of ovarian piRNAs, accumulation of transposable element transcripts, and female sterility. Conversely, Piwi depletion at later developmental stages does not disturb piRNA cluster licensing. These results indicate that the identity of piRNA clusters is epigenetically acquired in a Piwi-dependent manner during embryonic development, which is reminiscent of the widespread genome reprogramming occurring during early mammalian zygotic development.
Databáze: OpenAIRE
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