Impact of different patterns of sperm chromosomal abnormalities on the chromosomal constitution of preimplantation embryos
| Autor: | E. Mateu, José Remohí, Lorena Rodrigo, Carmen Rubio, Antonio Pellicer, Carlos Simón, Vanessa Peinado, Manuel Gil-Salom |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Gonadal dysgenesis Semen Biology Andrology Pregnancy medicine Humans In Situ Hybridization Fluorescence Infertility Male Preimplantation Diagnosis Sex Chromosome Aberrations Retrospective Studies Chromosome Aberrations medicine.diagnostic_test urogenital system Obstetrics and Gynecology Chromosome Karyotype Embryo Uniparental Disomy medicine.disease Diploidy Spermatozoa Sperm Semen Analysis Blastocyst Reproductive Medicine Gonadal Dysgenesis Mixed Female Ploidy Fluorescence in situ hybridization |
| Zdroj: | Fertility and Sterility. 94:1380-1386 |
| ISSN: | 0015-0282 |
| Popis: | Objective To evaluate the effect of sperm chromosome abnormalities—disomy for sex chromosomes and diploidy—in the chromosomal constitution of preimplantation embryos. Design Retrospective cohort study. Setting Infertility clinic. Patient(s) Three groups: 46,XY infertile men with increased incidence of sex chromosome disomy in sperm; 46,XY infertile men with increased diploidy rates in sperm; 47,XYY infertile men with increased sex chromosome disomy and diploidy rates in sperm. Intervention(s) Sperm collection for fluorescence in situ hybridization analysis. Embryo biopsy for preimplantation genetic screening. Main Outcome Measure(s) Frequencies of numerical abnormalities in sperm for chromosomes 13, 18, 21, X, and Y, and in embryos for chromosomes 13, 16, 18, 21, 22, X, and Y. Result(s) A significant increase of chromosomally abnormal and mosaic embryos was observed in the three study groups compared with controls. Those sperm samples with increased sex chromosome disomy rates produced significantly higher percentages of aneuploid embryos, with a threefold increase for sex chromosomes. Sperm samples with increased diploidy rates were mainly associated to the production of triploid embryos. Conclusion(s) A strong correlation between sperm and embryo chromosomal constitution has been shown in infertile men with 46,XY and 47,XYY karyotypes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|