Addendum: Kong, M.Y.; Whitley, R.J.; Peng, N.; Oster, R.; Schoeb, T.R.; Sullender, W.; Ambalavanan, N.; Clancy, J.P.; Gaggar, A.; Blalock J.E. Matrix Metalloproteinase-9 Mediates RSV Infection in Vitro and in Vivo. Viruses 2015, 30, 7, 4230–4253
| Autor: | J.E. Blalock, Ning Peng, Robert A. Oster, Trenton R. Schoeb, Namasivayam Ambalavanan, Amit Gaggar, Wayne M. Sullender, John P. Clancy, Richard J. Whitley, Michele Kong |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Smoking prevention
lcsh:QR1-502 Bioinformatics lcsh:Microbiology Virology Medicine Animals Humans Gene Silencing RNA Small Interfering Lung Cells Cultured Mice Knockout business.industry Addendum Matrix metalloproteinase 9 Epithelial Cells Viral Load 3. Good health Respiratory Syncytial Viruses Mice Inbred C57BL Infectious Diseases n/a Matrix Metalloproteinase 9 Host-Pathogen Interactions business |
| Zdroj: | Viruses Viruses, Vol 7, Iss 10, Pp 5609-5609 (2015) |
| ISSN: | 1999-4915 |
| Popis: | Respiratory Syncytial Virus (RSV) is an important human pathogen associated with substantial morbidity and mortality. The present study tested the hypothesis that RSV infection would increase matrix metalloproteinase (MMP)-9 expression, and that MMP-9 inhibition would decrease RSV replication both in vitro and in vivo. RSV A2 infection of human bronchial epithelial cells increased MMP-9 mRNA and protein release. Cells transfected with siRNA against MMP-9 following RSV infection had lower viral titers. In RSV infected wild-type (WT) mice, MMP-9, airway resistance and viral load peaked at day 2 post infection, and remained elevated on days 4 and 7. RSV infected MMP-9 knockout (KO) mice had decreased lung inflammation. On days 2 and 4 post inoculation, the RSV burden was lower in the MMP-9 KO mice compared to WT controls. In conclusion, our studies demonstrate that RSV infection is a potent stimulus of MMP-9 expression both in vitro and in vivo. Reduction of MMP-9 (via siRNA knockdown, and in MMP-9 KO mice) resulted in decreased viral replication. Our findings suggest MMP-9 is a potential therapeutic target for RSV disease. |
| Databáze: | OpenAIRE |
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