A Novel Tryptophanyl-tRNA Synthetase Gene Confers High-Level Resistance to Indolmycin▿ †
| Autor: | James J. Vecchione, Jason K. Sello |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Gene isoform
Indoles Molecular Sequence Data Streptomyces coelicolor Tryptophan-tRNA Ligase Tryptophan—tRNA ligase Drug resistance Microbial Sensitivity Tests Biology Mass Spectrometry Bacterial genetics Transcription (biology) Mechanisms of Resistance Drug Resistance Multiple Bacterial Pharmacology (medical) Amino Acid Sequence Peptide sequence Gene Pharmacology Genetics Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Point mutation Streptomyces griseus Tryptophan Streptomyces Anti-Bacterial Agents Isoenzymes Infectious Diseases Biochemistry Mutagenesis Site-Directed Chromatography Liquid |
| Popis: | Indolmycin, a potential antibacterial drug, competitively inhibits bacterial tryptophanyl-tRNA synthetases. An effort to identify indolmycin resistance genes led to the discovery of a gene encoding an indolmycin-resistant isoform of tryptophanyl-tRNA synthetase. Overexpression of this gene in an indolmycin-sensitive strain increased the indolmycin MIC 60-fold. Its transcription and distribution in various bacterial genera were assessed. The level of resistance conferred by this gene was compared to that of a known indolmycin resistance gene and to those of genes with resistance-conferring point mutations. |
| Databáze: | OpenAIRE |
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