Adherence Protects the Binding Sites ofHelicobacter pyloriUrease from Acid-Induced Damage
| Autor: | Hideo Goshima, Hideaki Yokoyama, Yutaka Ikemori, Chizu Kobayashi, Masahiko Kuroki, Faustino C. Icatlo, Yoshikatsu Kodama |
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| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Protein Denaturation Binding Sites Helicobacter pylori Urease Spirillaceae Immunology Mucin Mucins Hydrogen-Ion Concentration Biology Ligand (biochemistry) biology.organism_classification Microbiology Bacterial Adhesion Enzyme chemistry Virology biology.protein Binding site Bacteria |
| Zdroj: | Microbiology and Immunology. 44:773-776 |
| ISSN: | 0385-5600 |
| Popis: | Colonization by Helicobacter pylori partly depends on acid-dependent adherence by urease to gastric mucin. To further verify the relevance of urease adherence to colonization, the influence of acidity on the binding sites of H. pylori urease was investigated. When enzyme-based in vitro ligand capture assays were used, the effect of acidity on the binding site of H. pylori urease was determined against a backdrop medium consisting of acidic buffers simulating the luminal side of gastric mucus. A high degree of stability was exhibited by adherent urease, suggesting a pivotal role by the denatured enzyme in the persistence of the bacterium within the acidified compartment of gastric mucus. |
| Databáze: | OpenAIRE |
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