Identification of neuron-related genes for cell therapy of neurological disorders by network analysis* #
| Autor: | Lining Su, Huiping Wei, Hai-feng Yin, Xiaoqing Song |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Doublecortin Protein Cellular differentiation MAPK8 Bone Marrow Cells Biology General Biochemistry Genetics and Molecular Biology Article Transcriptome 03 medical and health sciences Protein Interaction Mapping Animals Cluster Analysis General Pharmacology Toxicology and Pharmaceutics Gene Cell Proliferation Oligonucleotide Array Sequence Analysis Genetics Regulation of gene expression Neurons General Veterinary Gene Expression Profiling Computational Biology SEMA3A Cell Differentiation Mesenchymal Stem Cells General Medicine Cell biology Rats Gene expression profiling 030104 developmental biology Gene Expression Regulation PAX6 Nervous System Diseases Software |
| Popis: | Bone mesenchymal stem cells (BMSCs) differentiated into neurons have been widely proposed for use in cell therapy of many neurological disorders. It is therefore important to understand the molecular mechanisms underlying this differentiation. We screened differentially expressed genes between immature neural tissues and untreated BMSCs to identify the genes responsible for neuronal differentiation from BMSCs. GSE68243 gene microarray data of rat BMSCs and GSE18860 gene microarray data of rat neurons were received from the Gene Expression Omnibus database. Transcriptome Analysis Console software showed that 1248 genes were up-regulated and 1273 were down-regulated in neurons compared with BMSCs. Gene Ontology functional enrichment, protein-protein interaction networks, functional modules, and hub genes were analyzed using DAVID, STRING 10, BiNGO tool, and Network Analyzer software, revealing that nine hub genes, Nrcam, Sema3a, Mapk8, Dlg4, Slit1, Creb1, Ntrk2, Cntn2, and Pax6, may play a pivotal role in neuronal differentiation from BMSCs. Seven genes, Dcx, Nrcam, sema3a, Cntn2, Slit1, Ephb1, and Pax6, were shown to be hub nodes within the neuronal development network, while six genes, Fgf2, Tgfβ1, Vegfa, Serpine1, Il6, and Stat1, appeared to play an important role in suppressing neuronal differentiation. However, additional studies are required to confirm these results. |
| Databáze: | OpenAIRE |
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