Platelet-activating Factor Induces Goblet Cell Hyperplasia and Mucin Gene Expression in Airways
| Autor: | Iris F. Ueki, Kiyoshi Takeyama, Ya-Ping Lou, Kathleen M. Grattan, Carlos Agustí, James A. Lausier, Jay A. Nadel |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty medicine.medical_treatment Guinea Pigs Bronchi Biology Critical Care and Intensive Care Medicine digestive system Pathogenesis chemistry.chemical_compound medicine Animals Platelet Activating Factor In Situ Hybridization Goblet cell Hyperplasia Mucous Membrane Platelet-activating factor Tumor Necrosis Factor-alpha Mucin Mucins respiratory system Asthma Rats Inbred F344 Epithelium Rats Trachea Cytokine medicine.anatomical_structure Gene Expression Regulation chemistry Tumor necrosis factor alpha Respiratory tract |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 157:1927-1934 |
| ISSN: | 1535-4970 1073-449X |
| Popis: | In patients dying from asthma, extensive mucous plugging occurs in the airways, associated with goblet cell hyperplasia. In this study, we examined the hypothesis that platelet-activating factor (PAF) induces goblet cell hyperplasia and mucin gene expression. After instilling PAF into the airways of guinea pigs and rats, we stained airway goblet cells with Alcian blue/periodic acid-Schiff and determined the number of goblet cells and percentage of stained area within the epithelium. In guinea pigs, one instillation of PAF (10(-)5 M, 100 microl) increased the goblet cell-stained area time-dependently, beginning at 24 h, maximum at 72 h. PAF also caused tracheal recruitment of eosinophils by 24 h, maximum at 48 h. In rats, which have few goblet cells in airways, PAF (3 instillations, 10(-)5 M, 200 microl) caused striking goblet cell hyperplasia, greatest in peripheral airways. Tumor necrosis factor alpha (TNFalpha) alone had no significant effect on goblet cells, but together with PAF, it caused exaggerated goblet cell hyperplasia. In rat tracheas studied by in situ hybridization, PAF induced mucin MUC5 gene expression in epithelial cells that stained for mucosubstances. In summary, PAF induces goblet cell hyperplasia and TNFalpha potentiates this effect. |
| Databáze: | OpenAIRE |
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