Prognostic Significance of miR-205 in Endometrial Cancer
| Autor: | Kenneth R. Shroyer, Jingfang Ju, Mihriban Karaayvaz, Sharon X. Liang, Cecilia Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
lcsh:Medicine
Gene Expression Biology In Vitro Techniques Bioinformatics medicine.disease_cause Molecular Genetics 03 medical and health sciences 0302 clinical medicine Breast cancer Uterine cancer Diagnostic Medicine Gene expression microRNA Molecular Cell Biology medicine Genetics Pathology Humans lcsh:Science 030304 developmental biology 0303 health sciences Multidisciplinary Endometrial cancer lcsh:R Computational Biology Cancers and Neoplasms medicine.disease Prognosis 3. Good health Endometrial Neoplasms MicroRNAs Oncology Tumor progression 030220 oncology & carcinogenesis Cancer research Medicine Cancer biomarkers lcsh:Q Female Carcinogenesis Gynecological Tumors Biomarkers Research Article General Pathology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 4, p e35158 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Purpose microRNAs have emerged as key regulators of gene expression, and their altered expression has been associated with tumorigenesis and tumor progression. Thus, microRNAs have potential as both cancer biomarkers and/or potential novel therapeutic targets. Although accumulating evidence suggests the role of aberrant microRNA expression in endometrial carcinogenesis, there are still limited data available about the prognostic significance of microRNAs in endometrial cancer. The goal of this study is to investigate the prognostic value of selected key microRNAs in endometrial cancer by the analysis of archival formalin-fixed paraffin-embedded tissues. Experimental Design Total RNAs were extracted from 48 paired normal and endometrial tumor specimens using Trizol based approach. The expression of miR-26a, let-7g, miR-21, miR-181b, miR-200c, miR-192, miR-215, miR-200c, and miR-205 were quantified by real time qRT-PCR expression analysis. Targets of the differentially expressed miRNAs were quantified using immunohistochemistry. Statistical analysis was performed by GraphPad Prism 5.0. Results The expression levels of miR-200c (P |
| Databáze: | OpenAIRE |
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