RETRACTED ARTICLE: Peroxidase Gene-Based Estimation of Genetic Relationships and Population Structure Among Wild Pistacia Species Populations
| Autor: | Setareh Mirzavand, Mohammad Reza Siahpoosh, Karim Sorkheh |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Germplasm Genetics Genetic diversity Pistacia 04 agricultural and veterinary sciences General Medicine Biology biology.organism_classification 01 natural sciences Biochemistry Genome DNA sequencing Genotype 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Gene family Molecular Biology Gene Ecology Evolution Behavior and Systematics 010606 plant biology & botany |
| Zdroj: | Biochemical genetics. 55(4) |
| ISSN: | 1573-4927 |
| Popis: | Plant peroxidases belong to a multigene family and possess highly conserved domains allowing designing oligonucleotide primers to amplify DNA sequences coding for peroxidases from plants with unsequenced genomes. Peroxidase gene-based polymorphism among wild Pistacia species, and correlation between peroxidase markers were deduced in this study. Wild Pistacia germplasm was evaluated using 30 peroxidase-specific primers. Targeted-PCR amplification of genomic DNA from 100 wild accessions of Pistacia species produced polymorphisms giving a similarity range from 0.29 to 1.00 with a mean of 0.65. Based on the POGP gene polymorphism, two distinct clusters were detected among the wild accession of Pistacia species, suggesting different evolutionary pathways. Correlation estimates as an indication of linkage disequilibrium between POGP markers ranged from 0.11 to 0.90, suggesting that few POGP markers were clustered and the remaining POGP markers evenly distributed throughout the Pistacia genome. These results demonstrate that primers targeting the peroxidase gene family can be used to study genotypic diversity and evolutionary relationships on an intra- and inter-specific basis. |
| Databáze: | OpenAIRE |
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