Hybrid hydrogel photonic barcodes for multiplex detection of tumor markers
| Autor: | Huan Wang, Yueshuang Xu, Yuanjin Zhao, Chengxin Luan, Baoan Chen, Xiao-Ping Zhang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Materials science
Biomedical Engineering Biophysics Nanotechnology 02 engineering and technology Biosensing Techniques 010402 general chemistry Barcode 01 natural sciences Hydrogel Polyethylene Glycol Dimethacrylate law.invention Polyethylene Glycols chemistry.chemical_compound law Limit of Detection Neoplasms Electrochemistry Biomarkers Tumor Humans Multiplex chemistry.chemical_classification Detection limit Immunoassay business.industry Biomolecule Sepharose Reproducibility of Results General Medicine Colloidal crystal 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Carcinoembryonic Antigen chemistry Agarose alpha-Fetoproteins Photonics 0210 nano-technology business Antibodies Immobilized Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 87 |
| ISSN: | 1873-4235 |
| Popis: | Barcodes-based suspension array have for demonstrated values in multiplex assay of tumor markers. Photonic barcodes which are encoded by their characteristic reflection peaks are the important supports for suspension array due to their stable code, low fluorescent background and high surface-volume ratio. Attempts to develop this technology tend to improve the function of the photonic barcodes. Here, we present a new type of hybrid hydrogel photonic barcodes for efficient multiplex assays. This photonic barcodes are hybrid inverse opal hydrogel composed of poly(ethylene glycol) diacrylate (PEG-DA) and agarose. The polymerized PEG-DA hydrogel could guarantee the stabilities of the inverse opal structure and its resultant code, while the agarose could offer active chemical groups for the probe immobilization and homogeneous water surrounding for the bioassay. In addition, the interconnected pores inverse opal structure could provide channels for biomolecules diffusing and reaction into the voids of barcodes. These features imparted the hybrid hydrogel photonic barcodes with limits of detection (LOD) of 0.78 ng/mL for carcinoembryonic antigen (CEA) and 0.21 ng/mL for α-fetoprotein (AFP), respectively. It was also demonstrated that the proposed barcodes showed acceptable accuracy and detection reproducibility, and the results were in acceptable agreement with those from common clinic method for the detections of practical clinical samples. Thus, our technique provides a new platform for simultaneous multiplex immunoassay. |
| Databáze: | OpenAIRE |
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