Radioiodinated antibody targeting of the HER-2/neu oncoprotein: effects of labeling method on cellular processing and tissue distribution
| Autor: | Yinhua Yu, Catherine F. Foulon, Fengji Xu, Robert C. Bast, Michael R. Zalutsky, Xiao-Guang Zhao, Susan Slade, Donna J. Affleck |
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| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
medicine.medical_specialty Cellobiose Immunoconjugates Receptor ErbB-2 medicine.drug_class Ratón media_common.quotation_subject Mice Nude Succinimides Tyramine Monoclonal antibody Iodine Radioisotopes Mice In vivo Internal medicine Tumor Cells Cultured medicine Animals Humans Urea Tissue Distribution Radiology Nuclear Medicine and imaging Internalization media_common Ovarian Neoplasms Mice Inbred BALB C biology Nicotinic Acids Antibodies Monoclonal medicine.disease Molecular biology In vitro Endocrinology Isotope Labeling biology.protein Molecular Medicine Female Antibody Ovarian cancer Intracellular |
| Zdroj: | Nuclear Medicine and Biology. 26:781-790 |
| ISSN: | 0969-8051 |
| DOI: | 10.1016/s0969-8051(99)00060-8 |
| Popis: | Monoclonal antibody (MAb) internalization can have a major effect on tumor retention of radiolabel. Two anti-HER-2/neu MAbs (TA1 and 520C9) were radioiodinated using the iodogen, N-succinimidyl 5-iodo-3-pyridinecarboxylate (SIPC), and tyramine-cellobiose (TCB) methods. Paired-label studies compared internalization and cellular processing of the labeled MAbs by SKOv3 9002-18 ovarian cancer cells in vitro. Intracellular radioiodine activity for 520C9 was up to 2.6 and 3.0 times higher for SIPC and TCB labeling, respectively, compared with iodogen. Likewise, intracellular activity for TA1 was up to 2.3 and 2.9 times higher with the SIPC and TCB methods compared with iodogen labeling. Unfortunately, similar advantages in tumor accumulation were not achieved in athymic mice bearing SKOv3 9008-18 ovarian cancer xenografts. |
| Databáze: | OpenAIRE |
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