An auxin-inducible, GAL4-compatible, gene expression system for Drosophila
| Autor: | Amira Hassan, Colin D McClure, Gabriel N Aughey, Khushbakht Butt, Alicia Estacio-Gómez, Aneisha Duggal, Chee Ying Sia, Annika F Barber, Tony D Southall |
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| Přispěvatelé: | Wellcome Trust |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
animal structures General Immunology and Microbiology biology ved/biology General Neuroscience Transgene fungi ved/biology.organism_classification_rank.species General Medicine 0601 Biochemistry and Cell Biology biology.organism_classification General Biochemistry Genetics and Molecular Biology Cell biology chemistry Auxin Gene expression Model organism Drosophila |
| Popis: | The ability to control transgene expression, both spatially and temporally, is essential for studying model organisms. In Drosophila, spatial control is primarily provided by the GAL4/UAS system, whilst temporal control relies on a temperature-sensitive GAL80 (which inhibits GAL4) and drug-inducible systems. However, these are not ideal. Shifting temperature can impact on many physiological and behavioural traits, and the current drug-inducible systems are either leaky, toxic, incompatible with existing GAL4-driver lines, or do not generate effective levels of expression. Here we describe the Auxin-inducible Gene Expression System (AGES). AGES relies on the auxin-dependent degradation of a ubiquitously expressed GAL80, and therefore, is compatible with existing GAL4-driver lines. Water-soluble auxin is added to fly food at a low, non-lethal, concentration, which induces expression comparable to uninhibited GAL4 expression. The system works in both larvae and adults, providing a stringent, non-lethal, cost-effective, and convenient method for temporally controlling GAL4 activity in Drosophila. |
| Databáze: | OpenAIRE |
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